+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Heat and protease treatment of aqueous humor: effect on cell DNA synthesis and growth



Heat and protease treatment of aqueous humor: effect on cell DNA synthesis and growth



Graefe's Archive for Clinical and Experimental Ophthalmology 222(3): 154-157



Rabbit skin and Tenon's capsule fibroblasts were cultured in media containing 20% rabbit aqueous humor as a stimulatory agent. Cell stimulation was quantitated by 3H-thymidine uptake. The aqueous humor was either: (1) untreated; (2) heated at 37 degrees C for 60 min; (3) boiled for 2 min; or (4) heated at 37 degrees C for 60 min with 0.025% trypsin, followed by boiling. The effects of the various treatments of aqueous humor on cell stimulation were compared among themselves and to 10% fetal bovine serum. It was found that: (1) heating of aqueous humor at 37 degrees C for up to 60 min had no apparent effect on its ability to stimulate DNA synthesis in culture; (2) boiling and trypsin plus boiling reduced the ability of 20% aqueous humor to promote thymidine uptake to varying degrees; (3) 20% untreated aqueous humor was more effective than 10% fetal bovine serum in stimulating DNA synthesis in Tenon's capsule fibroblasts; skin fibroblasts responded similarly to both media.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 040257813

Download citation: RISBibTeXText

PMID: 3979836

DOI: 10.1007/bf02173541


Related references

Antibodies to alpha B-crystallin, vimentin, and heat shock protein 70 in aqueous humor of patients with normal tension glaucoma and IgG antibody patterns against retinal antigen in aqueous humor. Current Eye Research 32(6): 501-509, 2007

Study of aqueous humor dynamic--aqueous humor fluorophotometry and fluorescein concentration curve of aqueous humor of the normal human eye. Chinese Journal of Ophthalmology 18(1): 17-23, 1982

Study of normal aqueous humor and aqueous humor in cases of glaucoma, and vitreous humor, by immunoelectrophoresis. Gazeta Medica Portuguesa 13: 649-651, 1960

Studies of aqueous humor dynamics: Part I. Effect of intravenous administration of beta blocking agent (propranolol) on tension lowering mechanism of rabbit eye as observed by determination of fluorescein concentration curve of aqueous humor. Chinese Journal of Ophthalmology 16(2): 113-116, 1980

Aqueous humor dynamics in glaucoma: effect of ganglionic blocking agents and thiopental sodium (pentothal) anesthesia on aqueous humor dynamics. A.M.A. Archives of Ophthalmology 55(6): 755-764, 1956

Effects of aqueous humor hydrodynamics on human eye heat transfer under external heat sources. Medical Engineering and Physics 38(8): 776-784, 2016

Trabecular cell expression of fibronectin and MMP-3 is modulated by aqueous humor growth factors. Experimental Eye Research 78(3): 653-660, 2004

Growth factors in secondary aqueous humor modulate trabecular cell expression of mmp 3. ARVO Annual Meeting Abstract Search & Program Planner : Abstract No 1167, 2003

Protease inhibitors and lectins in soya beans and effects of aqueous heat-treatment. Journal of the Science of Food & Agriculture 78(2): 225-231, 1998

The inhibitory effect of aqueous humor on the growth of cells in tissue cultures. American Journal of Ophthalmology 42(1): 70-74, 1956

The effect of aqueous humor on the growth of conjunctival fibroblasts in tissue culture. Investigative Ophthalmology & Visual Science: 125, 1977

Matrix metalloproteinases and protease inhibitors in the human aqueous humor. Molecular Biology of the Cell 3(Suppl. ): 318A, 1992

Effect of aqueous humor on apoptosis of inflammatory cell types. Investigative Ophthalmology & Visual Science 40(7): 1418-1426, 1999

Effect of irradiation on myeloid cell lysis of the aqueous humor. Medicina Em Revista 5: 25-28, 1960

Neuro-hormonal regulation of aqueous humor dynamics as the basis of glaucoma pharmacotherapy. Part I. Aqueous humor secretion. Klinika Oczna 109(1-3): 60-63, 2007