+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Hemagglutinin prepared from cells of Aedes albopictus, clone C6/36, infected with type 1 dengue virus



Hemagglutinin prepared from cells of Aedes albopictus, clone C6/36, infected with type 1 dengue virus



Biken Journal 22(2): 47-53



A high titer of hemagglutinin (HAnin) was found in culture fluids of cells of Aedes albopictus, clone C6/36, infected with type 1 dengue virus. The HAnin (TC antigen) was associated with complete virions and no appreciable small-sized HAnin was produced, in contrast to the case in an infected suckling mouse brain (SMB) homogenate, in which most of the HAnin is smaller than complete virions. Extraction of the TC antigen with Tween 80-ether disrupted the HAnin into smaller-sized particles, resulting in complete loss of infectivity (TE-TC antigen), but it did not affect the titer or reactivity of the HAnin. Comparative hemagglutination-inhibition (HI) assay of human sera using TC antigen, TE-TC antigen or standard antigen extracted from infected SMB (SMB antigen) showed that TC or TE-TC antigen could be used for routine diagnostic or epidemiological HI tests instead of SMB antigen, which is rather hard to prepare.

Please choose payment method:






(PDF emailed within 1 workday: $29.90)

Accession: 040260330

Download citation: RISBibTeXText

PMID: 533536


Related references

Phenotypic characteristics of dengue 2 virus persistently infected in a C6/36 clone of Aedes albopictus cells. Intervirology 37(1): 25-30, 1994

Electron microscopic examination of Aedes albopictus clone C6/36 cells infected with dengue virus 2 at elevated incubation temperature. Acta Virologica 42(1): 35-39, 1998

Synthesis of proteins and glycoproteins in dengue type 2 virus-infected vero and Aedes albopictus cells. Journal of General Virology 66: 559-571, 1985

Evaluation of Simultaneous Transmission of Chikungunya Virus and Dengue Virus Type 2 in Infected Aedes aegypti and Aedes albopictus (Diptera: Culicidae). Journal of Medical Entomology 52(3): 447-451, 2015

Resistance of dengue 1 virus infected cultured mosquito aedes albopictus cells to super infection by dengue 3 virus. Abstracts of the Annual Meeting of the American Society for Microbiology 81: 247, 1981

Amino acid requirements for the growth of Aedes albopictus clone C6/36 cells and for the production of dengue and Chikungunya viruses in the infected cells. Microbiology and Immunology 24(10): 915-924, 1980

Elevated incubation temperature enhanced antigen production of dengue type 2 and 3 viruses in the infected Aedes albopictus clone C6/36 cell cultures. Tropical Medicine 37(1): 21-27, 1995

Antigen production of dengue type 4 virus strains in Aedes albopictus clone C6/36 and vero cell cultures. Tropical Medicine 37(3): 115-121, 1995

The use of the C6/36 clone of Aedes albopictus cells for the development of live-attenuated dengue virus vaccines. Invertebrate cell system applications Volume II: 141-146, 1989

Changes in element content in Aedes albopictus infected by dengue virus type II. Chinese Journal of Parasitology and Parasitic Diseases 20(3): 171-173, 2002

Changes in element content in Aedes albopictus infected by dengue virus type II. Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi 20(3): 171-173, 2002

Dengue type 2 virus distribution in orally infected Aedes albopictus. Acta Parasitologica et Medica Entomologica Sinica 11(3): 157-160, 2004

Presence of an endogenous virus in uninfected Aedes albopictus cell, clone C6/36 Presenca de virus endogeno na linhagem celular Aedes albopictus clone C6/36 nao infectadas. Revista do Instituto Adolfo Lutz 572: 31-33, 1998

Dengue Type 2 Virus in Naturally Infected Aedes albopictus Mosquitoes in Singapore. Science 149(3684): 638-639, 1965

Studies on differential expressed genes of Aedes albopictus orally infected with dengue type 2 virus. Jishengchong Yu Yixue Kunchong Xuebao ; 11(2): 83-86, 2004