+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Human X-box-binding protein 1 is required for the transcription of a subset of human class II major histocompatibility genes and forms a heterodimer with c-fos



Human X-box-binding protein 1 is required for the transcription of a subset of human class II major histocompatibility genes and forms a heterodimer with c-fos



Proceedings of the National Academy of Sciences of the United States of America 88(10): 4309-4312



A complementary DNA encoding a member of the leucine-zipper class of proteins (human X-box-binding protein, hXBP-1) that binds to the 3' end of the conserved X box (X2) of the HLA-DRA major histocompatibility complex gene was recently described. Further gel-retardation analysis has demonstrated that hXBP-1 also binds to HLA-DPB X2 but not to other X2 sequences. Transient transfection of a mammalian expression vector with the hXBP-1 cDNA inserted in the antisense orientation represses the surface expression of HLA-DR and HLA-DP in Raji cells. Cotransfection of the antisense hXBP-1 vector with a HLA-DRA/chloramphenicol acetyltransferase (but not a HLA-DQB/chloramphenicol acetyltransferase) reporter plasmid decreases chloramphenicol acetyltransferase activity in Raji cells and in gamma-interferon-treated HeLa cells relative to cells cotransfected with a control antisense vector. Moreover, hXBP-1 is shown to form a stable heterodimer with the product of the c-fos protooncogene. These data suggest that the hXBP-1 c-fos heterodimer is critical for the transcription of a subset of the human class II major histocompatibility complex genes and that the regulatory mechanisms for the different class II genes are distinct.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 040310480

Download citation: RISBibTeXText

PMID: 1903538

DOI: 10.1073/pnas.88.10.4309


Related references

Transcription of a subset of human class II major histocompatibility complex genes is regulated by a nucleoprotein complex that contains c-fos or an antigenically related protein. Proceedings of the National Academy of Sciences of the United States of America 88(10): 4304-4308, 1991

Interactions between the class II transactivator and CREB binding protein increase transcription of major histocompatibility complex class II genes. Molecular and Cellular Biology 19(1): 941-947, 1999

Characterization of a major histocompatibility complex class II X-box-binding protein enhancing tat-induced transcription directed by the human immunodeficiency virus type 1 long terminal repeat. Journal of Virology 74(19): 8989-9001, 2000

Regulation of major histocompatibility (MHC) class II human leukocyte antigen-DR alpha gene expression in thyrocytes by single strand binding protein-1, a transcription factor that also regulates thyrotropin receptor and MHC class I gene expression. Endocrinology 139(5): 2300-2313, 1998

A novel cysteine-rich sequence-specific DNA-binding protein interacts with the conserved X-box motif of the human major histocompatibility complex class II genes via a repeated Cys-His domain and functions as a transcriptional repressor. Journal of Experimental Medicine 180(5): 1763-1774, 1994

Structure and expression of major histocompatibility complex-binding protein 2, a 275-kDa zinc finger protein that binds to an enhancer of major histocompatibility complex class I genes. Proceedings of the National Academy of Sciences of the United States of America 89(19): 8971-8975, 1992

Oligomerization of CD4 is required for stable binding to class II major histocompatibility complex proteins but not for interaction with human immunodeficiency virus gp120. Proceedings of the National Academy of Sciences of the United States of America 92(14): 6444-6448, 1995

The human major histocompatibility complex class II HLA-DRB1 and HLA-DQA1 genes are separated by a CTCF-binding enhancer-blocking element. Journal of Biological Chemistry 281(27): 18435-18443, 2006

Involvement of CREB binding protein in expression of major histocompatibility complex class II genes via interaction with the class II transactivator. Molecular and Cellular Biology 18(11): 6777-6783, 1998

Complexity of class i genes of the human major histocompatibility complex human class i major histocompatibility complex genes. Ziff, M And S B Cohen (Ed ) Advances in Inflammation Research, Vol 9 The Spondyloarthropathies Xv+272p Raven Press: New York, N Y , Usa Illus 25-34, 1985

Conserved upstream sequences of human class ii major histocompatibility genes enhance expression of class ii genes in wildtype but not mutant b cell lines. FASEB Journal 3(3): A519, 1989

Analysis of Class I Major Histocompatibility Complex Gene Transcription in Human Tumors Caused by Human Papillomavirus Infection. Viruses 9(9):, 2017

Conserved upstream sequences of human class II major histocompatibility genes enhance expression of class II genes in wild-type but not mutant B-cell lines. Proceedings of the National Academy of Sciences of the United States of America 85(21): 8186-8190, 1988

Transfer of cloned human class I major histocompatibility complex genes into HLA mutant human lymphoblastoid cells. Molecular and Cellular Biology 6(4): 1074-1087, 1986

Transcription specificity of the class Ib genes SLA-6, SLA-7 and SLA-8 of the swine major histocompatibility complex and comparison with class Ia genes. Animal Genetics 42(5): 510-520, 2011