Human allospecific TLCs generated against HLA antigens associated with DR1 through DRw8. I. Growth and specificity analysis

Rosen-Bronson, S.; Johnson, A.H.; Hartzman, R.J.; Eckels, D.D.

Immunogenetics 23(6): 368-378

1986


ISSN/ISBN: 0093-7711
PMID: 2941363
DOI: 10.1007/bf00372669
Accession: 040310605

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Abstract
To study the fine specificity of the HLA-D region, a panel of human T-lymphocyte clones (TLCs) was generated against alloantigens associated with HLA-DR1 through DRw8. HLA-DR-homozygous peripheral blood lymphocytes (PBLs) were stimulated with DR-heterozygous PBLs in primary mixed lymphocyte cultures for 4 days. Blasts were cloned by limiting dilution at 0.3 cells/well in the presence of 20% T-cell growth factor and irradiated stimulator cells. Viable clones were subsequently tested in proliferation assays against the original stimulator and a limited panel of stimulators bearing relevant DR specificities. Initial primings produced approximately 800 clones; some recognized DR-associated antigens, 70 recognized only their original stimulator, and approximately 50% were nonresponsive. Analysis on extended stimulator panels revealed alloantigenic complexity within similar DR-associated antigens as recognized by TLCs. The data are consistent with evidence that extreme heterogeneity exists within the HLA-D region.

Human allospecific TLCs generated against HLA antigens associated with DR1 through DRw8. I. Growth and specificity analysis