EurekaMag.com logo
+ Translate

Human chorionic gonadotropin: effects of crude and purified preparations on lymphocyte responses to phytohemagglutinin and allogenenic stimulation


, : Human chorionic gonadotropin: effects of crude and purified preparations on lymphocyte responses to phytohemagglutinin and allogenenic stimulation. Journal of Immunology 115(5): 1249-1253

The factors that prevent maternal immunologic rejection of the histoincompatible fetus are not understood. High levels of human chorionic gonadotropin (HCG) are present in the placenta, and several reports have noted suppresion of mitogen-induced lymphocyte transformation when cultures were supplemented with crude preparations of HCG. Purified HCG and multiple lots of crude HCG obtained from different suppliers were examined for their ability to suppress lymphocyte transformation produced by phytohemallutinin (PHA) or allogeneic stimulation. Crude preparations of HCG produced suppression of the lymphocyte stimulation induced by low doses of PHA, but the suppression could be overcome completely by increasing the PHA dose. The purified preparations of HCG produced no suppression of lymphocyte responses, even at the lower PHA dose. Purified HCG did not give a dose-related suppression of allogeneic lymphocyte responses, and crude lots of HCG gave highly variable results. One lot of crude HCG produced spontaneous stimulation of lymphocytes. Isoelectric focusing of HCG preparations demonstrated multiple bands, and lymphocyte suppression may be secondary to these additional unidentified proteins. The failure of pruified HCG to suppress lymphocyte responses makes it unlikely that the absence of maternal rejection of the fetus is due to high placental levels of HCG.

(PDF 0-2 workdays service)

Accession: 040311627

PMID: 126260

Submit PDF Full Text: Here


Submit PDF Full Text

No spam - Every submission is manually reviewed

Due to poor quality, we do not accept files from Researchgate

Submitted PDF Full Texts will always be free for everyone
(We only charge for PDFs that we need to acquire)

Select a PDF file:
Close
Close

Related references

Caldwell J.L.; Stites D.P.; Fudenberg H.H., 1975: Human chorionic gonadotropin effects of crude and purified preparations on lymphocyte responses to phyto hem agglutinin and allogeneic stimulation. Journal of Immunology 115(5): 1249-1253

Gundert, D.; Merz, W.E.; Hilgenfeldt, U.; Brossmer, R., 1975: Inability of highly purified preparations of human chorionic gonadotropin to inhibit the phytohemagglutinin-induced stimulation of lymphocytes. Febs Letters 53(3): 309-312

Schmidt, W.; Merz, W.E.; Lenhard, V.; Kubli, F., 1981: Effects of crude and purified human chorionic gonadotropin on lymphocyte response. Human chorionic gonadotropin (hCG) preparations with different biological activities were tested for their inhibitory effects on mitogenic of allogenic induced lymphocyte response. Various crude hormone batches inhibited the lymphocyte reaction in...

Pattillo, R.A.; Shalaby, M.R.; Hussa, R.O.; Bahl, O.P.; Mattingly, R.F., 1976: Effect of crude and purified human chorionic gonadotropin on lymphocyte blastogenesis. Crude APL [anterior pituitary-like hormone] hCG [human chorionic gonadotropin] completely inhibited blastogenesis induced by phytohemagglutinin, pokeweed mitogen or concanavalin A in normal [human] lymphocytes and lymphocytes obtained from cancer...

Amir S.M.; Sullivan R.C.; Ingbar S.H., 1980: In vitro responses to crude and purified human chorionic gonadotropin in human thyroid membranes. Human chorionic gonadotropin [hCG] has intrinsic thyrotropic activity in the mouse. To obtain evidence of whether hCG has stimulatory activity in the human thyroid, the interactions of crude and pure hCG with receptors in human thyroid membranes w...

Maes, R.F.; Claverie, N., 1977: The effect of preparations of human chorionic gonadotropin on lymphocyte stimulation and immune response. Optimal concentrations of human chorionic gonadotropin (HCG) enhance the uptake of 3H-thymidine (TdR) by nu/nu and BALB spleen cells maintained in tissue culture. The enhancing effect of HCG is found with crude and pure hormone. At higher concentr...

Anonymous, 2007: Effect of highly purified human chorionic gonadotropin preparations on endometriotic lesions: potential mechanisms for the therapeutic effect of human chorionic gonadotropin in vivo

Huber, A.; Hudelist, G.; Knöfler, M.; Saleh, L.; Huber, J.C.; Singer, C.F., 2007: Effect of highly purified human chorionic gonadotropin preparations on the gene expression signature of stromal cells derived from endometriotic lesions: potential mechanisms for the therapeutic effect of human chorionic gonadotropin in vivo. Objective: To investigate alterations in the overall gene expression profile of endometriosis-derived stroma with increasing concentrations of hCG by using the Affymetrix GeneChip U133 Set.Design: In vitro study. Setting: Academic research institu...

Huhtaniemi, I.T.; Catt, K.J., 1985: Effects of gonadotropin-releasing hormone receptor blockade on rat testicular gonadotropin and lactogen receptors, steroidogenesis, and responses to human chorionic gonadotropin stimulation. Testicular endocrine regulation was studied in adult male rats after treatment with a potent GnRH antagonist analog [N-Ac-D-p-Cl-Phe1,2, D-Trp3, D-Lys6, D-Ala10-GnRH (Ant.)] given in doses of 1 mg/kg at 0, 12, and 24 h. One group of animals also r...

Morse, J.H.; Stearns, G.; Arden, J.; Agosto, G.M.; Canfield, R.E., 1976: The effects of crude and purified human gonadotropin on in vitro stimulated human lymphocyte cultures. Crude human chorionic gonadotropin (hCG) was several fold more immunosuppressive than purified hCG in human peripheral blood lymphocyte cultures stimulated by phytohemagglutinin, pokeweed [mitogen], purified protein derivative and allogeneic cells...