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Human enteric adenovirus type 41 binding to HEp-2 cells is specific

Human enteric adenovirus type 41 binding to HEp-2 cells is specific

Virology 183(1): 410-414

Properties of human enteric adenovirus type 41 (Ad41) binding to its receptor on the surface of HEp-2 cells were investigated. The binding was found to be temperature-dependent, saturable, and specific. Analysis of the binding data showed a single class of 4.3 x 10(4) receptor sites per cell, an equilibrium dissociation constant of 21.0 nM, and no cooperativity among receptor sites. Trypsin-treated HEp-2 cells subsequently grown in the presence of tunicamycin or 2-deoxyglucose recovered full Ad41 binding activity, but could not if subsequently grown in the presence of cycloheximide. These data indicate that a single type of virus receptor, likely protein in nature, is present on the surface of HEp-2 cells to specifically bind Ad41.

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Accession: 040312194

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PMID: 2053291

DOI: 10.1016/0042-6822(91)90156-6

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