+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Human growth hormone in the blood of athymic mice grafted with cultures of hormone-secreting human keratinocytes



Human growth hormone in the blood of athymic mice grafted with cultures of hormone-secreting human keratinocytes



Faseb Journal 4(14): 3245-3250



An established line of human epidermal keratinocytes was permanently transformed by transfection with plasmid vectors bearing the human growth hormone gene under the control of two different promoters. Suitable selection permitted the isolation of clones secreting abundant growth hormone in culture. When such cultures were grafted to athymic mice, human growth hormone could be detected in the blood of the mice at concentrations in the physiological range for more than 4 weeks. Retrograde transfer from epithelial cells should be studied further as a possible means of introducing exogenous gene products into the circulation of humans.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 040312952

Download citation: RISBibTeXText

PMID: 2227214

DOI: 10.1096/fasebj.4.14.2227214


Related references

Autoradiographic demonstration of in vivo iodine growth hormone releasing hormone ghrh binding by human gh secreting pituitary adenomas transplanted on athymic nude mice. Molecular & Cellular Endocrinology 85(3): 157-164, 1992

Autoradiographic demonstration of in vivo 125I-growth hormone-releasing hormone (GHRH) binding by human GH-secreting pituitary adenomas transplanted on athymic nude mice. Molecular and Cellular Endocrinology 85(3): 157-164, 1992

Use of athymic nude mice for in vivo studies of human growth-hormone-secreting pituitary adenomas. Hormone Research 35(5): 198-204, 1991

Increases in weight of growth hormone-deficient and immunodeficient (lit/scid) dwarf mice after grafting of hGH-secreting, primary human keratinocytes. Faseb Journal 17(15): 2322-2324, 2003

Absence of response to human parathyroid hormone in athymic mice grafted with human parathyroid adenoma, hyperplasia or parathyroid cells maintained in culture. Journal of Endocrinological Investigation 23(5): 273-279, 2000

Human growth hormone releasing factor (hGRF) modulates calcium currents in human growth hormone secreting adenoma cells. Brain Research 604(1-2): 345-347, 1993

Inverse relation between mRNA synthesis and secretion of parathyroid hormone in athymic mice grafted with human parathyroid tissue. APMIS 109(3): 135-240, 2001

Growth response in athymic nude mice to transplanted growth hormone-secreting rat pituitary tumor cells. Endocrinology 103(1): 223-228, 1978

Assessment of replication rates of human keratinocytes in engineered skin substitutes grafted to athymic mice. Wound Repair and Regeneration 20(4): 544-551, 2012

Transgenic mice expressing the human growth hormone gene provide a model system to study human growth hormone synthesis and secretion in non-tumor-derived pituitary cells: differential effects of dexamethasone and thyroid hormone. Molecular and Cellular Endocrinology 345(1-2): 48-57, 2011

Immunological behavior of human growth hormone from plasma of patients with familial dwarfism and high immuno reactive human growth hormone in a radio immunoassay system using the cross reaction between human growth hormone and human chorionic somatomammotropin. Hormone & Metabolic Research 6(1): 80-81, 1974

On the effect of synthetic luteinizing hormone releasing hormone on the blood levels of luteinizing hormone follicle stimulating hormone human growth hormone thyroid stimulating hormone acth insulin and blood sugar in man. Hormone & Metabolic Research 4(5): 403, 1972

Immunochemical studies on human growth hormone: A consideration of the human growth hormone-anti-human growth hormone system and its application to the assay of growth hormone. Ciba Found Colloquia Endocrinol 14: 63-104, 1962

Site-selective growth of a hormone-responsive human breast carcinoma in athymic mice. Cancer Research 42(3): 906-912, 1982

Inhibitory effect of bombesin/gastrin-releasing peptide antagonist RC-3095 and luteinizing hormone-releasing hormone antagonist SB-75 on the growth of MCF-7 MIII human breast cancer xenografts in athymic nude mice. Cancer (philadelphia). 73(4): 1229-1238, 1994