+ Site Statistics
References:
54,258,434
Abstracts:
29,560,870
PMIDs:
28,072,757
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Hyperplasia of mouse mammary epithelium induced by expression of the Wnt-1 (int-1) oncogene in reconstituted mammary gland



Hyperplasia of mouse mammary epithelium induced by expression of the Wnt-1 (int-1) oncogene in reconstituted mammary gland



Oncogene 7(10): 2041-2051



We have expressed the Wnt-1 (formerly int-1) oncogene in Balb/c mouse mammary epithelium in vivo, using a tissue reconstitution method in which primary cultures of mammary epithelial cells are infected with a retrovirus vector and then transplanted into mouse mammary fat pads from which the natural epithelium has been removed. Transplants carrying the Wnt-1 gene grew in a hyperplastic pattern, the duct epithelium showing abundant fine side-branches, but without development of clusters of alveoli. The hyperplasias were similar, but not identical, to transplants of normal epithelium in a mid-pregnant host. Transplants of epithelium that expressed Wnt-1 into mammary fat pads of male or ovariectomized females grew to form a similar three-dimensional pattern, but the extent of growth, and so presumably the rate of growth, was slower than in intact females, and there were no terminal end buds at the edges of the outgrowths. Thus, although Wnt-1 may enhance growth of epithelium in the male or ovariectomized-female environment, it does not restore the major mode of growth in the intact female, the extension of major ducts from terminal end buds. Normal epithelium showed no change in morphology when in close proximity to hyperplasia induced by Wnt-1, confirming the limited range of diffusion of Wnt-1 protein in vivo. Our results are consistent with the hypothesis that Wnt-1 acts principally by mimicking the signal that causes ducts to develop side-branches in pregnancy.

Please choose payment method:






(PDF emailed within 1 workday: $29.90)

Accession: 040326049

Download citation: RISBibTeXText

PMID: 1408145


Related references

Induction of epithelial abnormalities that resemble human breast lesions by the expression of the neu/erbB-2 oncogene in reconstituted mouse mammary gland. Oncogene 8(6): 1551-1558, 1993

Modulation in the expression of murine mammary tumor virus, ras proto-oncogene, and of alveolar hyperplasia by fatty acids in mouse mammary explant cultures. Anticancer Research 8(5a): 971-976, 1988

H ras induced transformation of mammary epithelium is favored by increased oncogene expression or by inhibition of mammary regression. Oncogene 6(5): 771-780, 1991

Whey acidic protein extrinsically expressed from the mouse mammary tumor virus long terminal repeat results in hyperplasia of the coagulation gland epithelium and impaired mammary development. Cell Growth and Differentiation 5(6): 607-613, 1994

Inappropriate P-cadherin expression in the mouse mammary epithelium is compatible with normal mammary gland function. Differentiation; Research in Biological Diversity 71(6): 361-373, 2003

TBX3 over-expression causes mammary gland hyperplasia and increases mammary stem-like cells in an inducible transgenic mouse model. Bmc Developmental Biology 11: 65, 2012

Conditional loss of ErbB3 delays mammary gland hyperplasia induced by mutant PIK3CA without affecting mammary tumor latency, gene expression, or signaling. Cancer Research 73(13): 4075-4085, 2013

The expression of N-terminally truncated occludin causes programmed cell death in the mouse mammary gland epithelium and in a mammary epithelial cell line. Molecular Biology of the Cell 12(Supplement): 418a, 2001

Studies on the mouse mammary tumor agent mta part 2 cytochemistry of amino peptidase in normal mammary gland of c 57 bl spontaneous and virus induced mammary tumors in r 111 and c 57 bl mouse strains respectively and in cell lines producing the mammary tumor virus. Acta Histochemica: 181-188, 1970

Epimorphin overexpression in the mouse mammary gland promotes alveolar hyperplasia and mammary adenocarcinoma. Cancer Research 65(19): 8617-8621, 2005

STAT3 expression in canine mammary gland tumours and mammary gland hyperplasia. European Journal of Veterinary Pathology 7(3): 117-122, 2001

Studies on the mouse mammary tumour agent (MTA). IV. Lactate and malate dehydrogenase isozymes in normal mammary gland of R3f, C57BL and in virus-induced and spontaneous mammary adenocarcinoma in C57BL and R3 mouse strains. Acta Histochemica 46(1): 136-139, 1973

Studies on the mouse mammary tumour agent (MTA). II. Cytochemistry of aminopeptidase in normal mammary gland of C57BL, spontaneous and virus-induced mammary tumours in RIII and C57BL mouse strains, respectively, and in cell lines producing the mammary tumour virus. Acta histochem, 38: 1, 181-88, 1970

Studies on the mouse mammary tumour agent (MTA). II. Cytochemistry of aminopeptidase in normal mammary gland of C57BL, spontaneous and virus-induced mammary tumours in R3 and C57BL mouse strains, respectively, and in cell lines producing the mammary tumour virus. Acta Histochemica 38(1): 181-188, 1970

A mouse mammary tumor virus-Wnt-1 transgene induces mammary gland hyperplasia and tumorigenesis in mice lacking estrogen receptor-alpha. Cancer Research 59(8): 1869-1876, 1999