+ Most Popular
Cunninghamia lanceolata plantations in China
Mammalian lairs in paleo ecological studies and palynology
Studies on technological possibilities in utilization of anhydrous milk fat for production of recombined butter-like products
Should right-sided fibroelastomas be operated upon?
Large esophageal lipoma
Apoptosis in the mammalian thymus during normal histogenesis and under various in vitro and in vivo experimental conditions
Poissons characoides nouveaux ou non signales de l'Ilha do Bananal, Bresil
Desensitizing efficacy of Colgate Sensitive Maximum Strength and Fresh Mint Sensodyne dentifrices
Administration of fluid by subcutaneous infusion: revival of a forgotten method
Tundra mosquito control - an impossible dream?
Schizophrenia for primary care providers: how to contribute to the care of a vulnerable patient population
Geochemical pattern analysis; method of describing the Southeastern limestone regional aquifer system
Incidence of low birth weights in a hospital of Mexico City
Graded management intensity of grassland systems for enhancing floristic diversity
Microbiology and biochemistry of cheese and fermented milk
The ember tetra: a new pygmy characid tetra from the Rio das Mortes, Brazil, Hyphessobrycon amandae sp. n. (Pisces, Characoidei)
Risk factors of contrast-induced nephropathy in patients after coronary artery intervention
Renovation of onsite domestic wastewater in a poorly drained soil
Observations of the propagation velocity and formation mechanism of burst fractures caused by gunshot
Systolic blood pressure in a population of infants in the first year of life: the Brompton study
Haematological studies in rats fed with metanil yellow
Studies on pasteurellosis. I. A new species of Pasteurella encountered in chronic fowl cholera
Dormancy breaking and germination of Acacia salicina Lindl. seeds
therapy of lupus nephritis. a two-year prospective study

Identification of an alternatively spliced site in human plasma fibronectin that mediates cell type-specific adhesion

Identification of an alternatively spliced site in human plasma fibronectin that mediates cell type-specific adhesion

Journal of Cell Biology 103(6 Part 2): 2637-2647

ISSN/ISBN: 0021-9525

PMID: 3025221

DOI: 10.1083/jcb.103.6.2637

We have compared the molecular specificities of the adhesive interactions of melanoma and fibroblastic cells with fibronectin. Several striking differences were found in the sensitivity of the two cell types to inhibition by a series of synthetic peptides modeled on the Arg-Gly-Asp-Ser (RGDS) tetrapeptide adhesion signal. Further evidence for differences between the melanoma and fibroblastic cell adhesion systems was obtained by examining adhesion to proteolytic fragments of fibronectin. Fibroblastic BHK cells spread readily on fl3, a 75-kD fragment representing the RGDS-containing, "cell-binding" domain of fibronectin, but B16-F10 melanoma cells could not. The melanoma cells were able to spread instead on f9, a 113-kD fragment derived from the large subunit of fibronectin that contains at least part of the type III connecting segment difference region (or "V" region); f7, a fragment from the small fibronectin subunit that lacks this alternatively spliced polypeptide was inactive. Monoclonal antibody and fl3 inhibition experiments confirmed the inability of the melanoma cells to use the RGDS sequence; neither molecule affected melanoma cell spreading, but both completely abrogated fibroblast adhesion. By systematic analysis of a series of six overlapping synthetic peptides spanning the entire type III connecting segment, a novel attachment site was identified in a peptide near the COOH-terminus of this region. The tetrapeptide sequence Arg-Glu-Asp-Val (REDV), which is somewhat related to RGDS, was present in this peptide in a highly hydrophilic region of the type III connecting segment. REDV appeared to be functionally important, since this synthetic tetrapeptide was inhibitory for melanoma cell adhesion to fibronectin but was inactive for fibroblastic cell adhesion. REDV therefore represents a novel adhesive recognition signal in fibronectin that possesses cell type specificity. These results suggest that, for some cell types, regulation of the adhesion-promoting activity of fibronectin may occur by alternative mRNA splicing.

Please choose payment method:

(PDF emailed within 0-6 h: $19.90)

Accession: 040341437

Download citation: RISBibTeXText

Related references

Identification of two distinct regions of an alternatively spliced site in human plasma fibronectin that promotes cell type specific adhesion. Marshall, G R (Ed ) Peptides: Chemistry And Biology; Tenth American Peptide Symposium, St Louis, Missouri, Usa, May 23-28, 1987 Xxxiii+690p Escom Science Publishers B V : Leiden, Netherlands Illus 632-634, 1988

Identification of a new cell type specific adhesion signal alternatively spliced in human plasma fibronectin. Journal of Cell Biology 103(5 Part 2): 89A, 1986

Detailed characterization of a major cell type specific adhesion site within the alternatively spliced iiics domain of fibronectin. Journal of Cell Biology 107(6 Part 3): 384A, 1988

The minimal essential sequence for a major cell type specific adhesion site cs1 within the alternatively spliced iiics domain of fibronectin is leu asp val. FASEB Journal 5(6): A1617, 1991

The minimal essential sequence for a major cell type-specific adhesion site (CS1) within the alternatively spliced type III connecting segment domain of fibronectin is leucine-aspartic acid-valine. Journal of Biological Chemistry 266(23): 15075-15079, 1991

Identification of a novel heparin-binding site in the alternatively spliced IIICS region of fibronectin: Roles of integrins and proteoglycans in cell adhesion to fibronectin splice variants. Matrix Biology: Journal of the International Society for Matrix Biology 20(1): 63-73, 2001

Effect of cell binding domain and alternatively spliced region of human plasma fibronectin on adhesion to resting U937 cells. Yokohama Medical Bulletin 46(3-4): 89, 1995

Cell-type-specific expression of alternatively spliced human fibronectin IIICS mRNAs. Molecular and Cellular Biology 10(2): 662-671, 1990

Neurite extension of chicken peripheral nervous system neurons on fibronectin: relative importance of specific adhesion sites in the central cell-binding domain and the alternatively spliced type III connecting segment. Journal of Cell Biology 106(4): 1289-1297, 1988

Neurite Extension of Chicken Peripheral Nervous System Neurons on Fibronectin: Relative Importance of Specific Adhesion Sites in the Central Cell-Binding Domain and the Alternatively Spliced Type Iii Connecting Segment. The Journal of Cell Biology 106(4): 1289-1297, 1988

Adhesion activity in fibronectin's alternatively spliced domain EDa (EIIIA): complementarity to plasma fibronectin functions. Experimental Cell Research 217(2): 517-527, 1995

The alternatively spliced domains EIIIB and EIIIA of human fibronectin affect cell adhesion and spreading. Journal of Cell Science 110: 2271-2280, 1997

Identification of two distinct regions of the type III connecting segment of human plasma fibronectin that promote cell type-specific adhesion. Journal of Biological Chemistry 262(14): 6886-6892, 1987

Site-specific HNK-1 epitope on alternatively spliced fibronectin type-III repeats in tenascin-C promotes neurite outgrowth of hippocampal neurons through contactin-1. Plos one 14(1): E0210193, 2019

Tenascin-C Promotes Neurite Outgrowth of Embryonic Hippocampal Neurons through the Alternatively Spliced Fibronectin Type Iii Bd Domains via Activation of the Cell Adhesion Molecule F3/Contactin. The Journal of Neuroscience 22(15): 6596-6609, 2002