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Identification of functional regions in the C-terminal domain of Escherichia coli ribosomal protein S1 using monoclonal antibodies



Identification of functional regions in the C-terminal domain of Escherichia coli ribosomal protein S1 using monoclonal antibodies



Biochemical and Biophysical Research Communications 149(1): 34-39



Monoclonal antibodies specific for defined regions of E. coli ribosomal protein S1 were used in a R17 mRNA-directed protein synthesis assay to reveal functionally important sites of the protein. Two distinct sites for mRNA binding were identified in the regions 349-437 and 438-547 located in the C-terminal domain of protein S1.

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Accession: 040342497

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PMID: 2446623

DOI: 10.1016/0006-291x(87)91601-9


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