EurekaMag
+ Most Popular
Cunninghamia lanceolata plantations in China
Mammalian lairs in paleo ecological studies and palynology
Studies on technological possibilities in utilization of anhydrous milk fat for production of recombined butter-like products
Should right-sided fibroelastomas be operated upon?
Large esophageal lipoma
Apoptosis in the mammalian thymus during normal histogenesis and under various in vitro and in vivo experimental conditions
Poissons characoides nouveaux ou non signales de l'Ilha do Bananal, Bresil
Desensitizing efficacy of Colgate Sensitive Maximum Strength and Fresh Mint Sensodyne dentifrices
Administration of fluid by subcutaneous infusion: revival of a forgotten method
Tundra mosquito control - an impossible dream?
Schizophrenia for primary care providers: how to contribute to the care of a vulnerable patient population
Geochemical pattern analysis; method of describing the Southeastern limestone regional aquifer system
Incidence of low birth weights in a hospital of Mexico City
Tabanidae
Graded management intensity of grassland systems for enhancing floristic diversity
Microbiology and biochemistry of cheese and fermented milk
The ember tetra: a new pygmy characid tetra from the Rio das Mortes, Brazil, Hyphessobrycon amandae sp. n. (Pisces, Characoidei)
Risk factors of contrast-induced nephropathy in patients after coronary artery intervention
Renovation of onsite domestic wastewater in a poorly drained soil
Observations of the propagation velocity and formation mechanism of burst fractures caused by gunshot
Systolic blood pressure in a population of infants in the first year of life: the Brompton study
Haematological studies in rats fed with metanil yellow
Studies on pasteurellosis. I. A new species of Pasteurella encountered in chronic fowl cholera
Dormancy breaking and germination of Acacia salicina Lindl. seeds
therapy of lupus nephritis. a two-year prospective study

Identification of sequences in Epstein-Barr virus DNA required for the expression of the second Epstein-Barr virus-determined nuclear antigen in COS-1 cells


Identification of sequences in Epstein-Barr virus DNA required for the expression of the second Epstein-Barr virus-determined nuclear antigen in COS-1 cells



Journal of General Virology 68: 2407-2418



ISSN/ISBN: 0022-1317

PMID: 2821180

DOI: 10.1099/0022-1317-68-9-2407

The BamHI WYH region of the Epstein-Barr virus (EBV) genome encodes a protein localized to the nucleus of the infected cell, the EBV-determined nuclear antigen EBNA2. We have constructed a series of recombinant vectors that carried the complete EBNA2 gene, or the gene modified so as to contain defined deletions involving presumed exons and regulatory elements of the gene. The recombinant vectors were transfected into COS-1 cells which permit the replication of simian virus 40 origin-containing plasmids to a high copy number, and the transient expression of EBNA2 was analysed. A recombinant plasmid that carried a BglII-NotI subfragment of the BamHI WYH region (nucleotides 44664 to 50628) contained all the information necessary for inducing the expression of a full length EBNA2 polypeptide. Moreover, EBV DNA sequences between nucleotides 45,442 and 48,337 could be deleted without interfering with the ability of the vectors to induce EBNA2. On the other hand the loss of the left one-third of the BglII-NotI fragment completely abolished the EBNA2-inducing capacity of the vector. A rightward promoter consensus sequence in the BamHI W part of the BglII-NotI fragment was functional in COS-1 cells expressing EBNA2 and essential for EBV-specific RNA synthesis. The results indicated that transcription of the EBNA2 gene was initiated in the BamHI W fragment, that the transcript was spliced and that all of EBNA2 was encoded within the continuous long open reading frame in the BamHI Y and H fragments.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 040344075

Download citation: RISBibTeXText

Related references

Identification of sequences in epstein barr virus dna required for the expression of the second epstein barr virus determined nuclear antigen. Uicc (union Internationale Contre Le Cancer, International Union Against Cancer)international Cancer Congress, Budapest, Hungary: 21-27,: Basel, Switzerland; New York, N.y., Usa; Akademiai Kiado: Budapest, Hungary. Paper. 817, 1986

Relationship between the amounts of epstein barr virus dna and epstein barr virus nuclear antigen per cell clonability and tumorigenicity in 2 epstein barr virus negative lymphoma lines and their epstein barr virus converted sublines. International Journal of Cancer 31(2): 163-170, 1983

Transfection of epstein barr virus dna fragments coding for epstein barr virus nuclear antigen 1 and epstein barr virus nuclear antigen 2 into human lymphocytes gene expression without cell immortalization. Journal of Cellular Biochemistry Suppl (10 Part A): 213, 1986

Establishment of epstein barr virus nuclear antigen expressing cell lines by infection of epstein barr virus genome negative human lymphoma cells with different epstein barr virus strains. International Journal Of Cancer: 161-166, 1976

Epstein-barr virus nuclear antigen 3C activates the latent membrane protein 1 promoter in the presence of Epstein-Barr virus nuclear antigen 2 through sequences encompassing an spi-1/Spi-B binding site. Journal of Virology 74(11): 5151-5160, 2000

Effects of S-adenosylhomocysteine and analogs on Epstein-Barr virus-induced transformation, expression of the Epstein-Barr virus capsid antigen, and methylation of Epstein-Barr virus DNA. Journal of Virology 61(1): 221-224, 1987

Expression of the Epstein-Barr virus-encoded Epstein-Barr virus nuclear antigen 1 in Hodgkin's lymphoma cells mediates Up-regulation of CCL20 and the migration of regulatory T cells. American Journal of Pathology 173(1): 195-204, 2008

Purification of the Epstein-Barr virus-determined nuclear antigen from Epstein-Barr virus-transformed human lymphoid cell lines. Journal of Virology 27(3): 604-611, 1978

Studies on the epstein barr virus genome and the epstein barr virus determined nuclear antigen in human malignant disease. Ford, Nancy (Ed ) Cold Spring Harbor Symposia on Quantitative Biology, Vol 39 Tumor Viruses Xxvi+688p (Vol 1 ); Xvi+545p (Vol 2) Illus Cold Spring Harbor Laboratory: Cold Spring Harbor, N Y, U S A ISBN 0-87969-038-0: 783-790, 1975

Selection of an epstein barr nuclear antigen positive variant from an epstein barr nuclear antigen negative human lympho blastoid cell line without detectable epstein barr virus dna. Journal of Medical Microbiology 8(2): PXII, 1975

Epstein-Barr virus nuclear antigen 2 function is required for maintenance of Epstein-Barr virus induced B-cell transformation. Journal of Cellular Biochemistry Suppl 1994(18C): 231, 1994

Efficient expression of an Epstein-Barr nuclear antigen in Drosophila cells transfected with Epstein-Barr virus Dna. The Embo Journal 4(11): 2955-2959, 1985

Efficient expression of an Epstein-Barr nuclear antigen in Drosophila cells transfected with Epstein-Barr virus DNA. Embo Journal 4(11): 2955-2959, 1985

Molecular size variation of epstein barr virus induced nuclear antigen is determined by the epstein barr viral genome. Acta Microbiologica Hungarica 31(3): 259-260, 1984

Constitutive expression of Epstein-Barr virus-encoded RNAs and nuclear antigen during latency and after induction of Epstein-Barr virus replication. Journal of Virology 53(1): 254-259, 1985