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Immunofluorescence determination of IgG following high performance liquid gel permeation chromatography

Immunofluorescence determination of IgG following high performance liquid gel permeation chromatography

Journal of Immunological Methods 85(1): 115-119

ISSN/ISBN: 0022-1759

PMID: 3908558

DOI: 10.1016/0022-1759(85)90279-0

The application of high performance liquid gel permeation chromatography (HPL-GPC) and immunofluorescence for the estimation of a protein was studied using human IgG as a model system following separation of the fluorescent immune complex from free fluorescent-labelled antibody on a column of HPL-GPC. Using FITC-labelled anti-human IgG Fab fragment and HPL-GPC, nanogram levels of IgG could be determined within 5 min of mixing the antigen and antiserum. HPL-GPC immunofluorescence analysis is sensitive specific, simple, rapid and highly versatile.

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Accession: 040359693

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