EurekaMag
+ Translate
+ Most Popular
The pigeon tick (Argas reflexus): its biology, ecology, and epidemiological aspects
Prevalence of hemoglobin abnormalities in Kebili (Tunisian South)
Lipogranuloma: a preventable complication of dacryocystorhinostomy
Value of basal plasma cortisol assays in the assessment of pituitary-adrenal insufficiency
Bees from the Belgian Congo. The acraensis group of Anthophora
Placing gingival retraction cord
Total serum IgE, allergy skin testing, and the radioallergosorbent test for the diagnosis of allergy in asthmatic children
Acariens plumicoles Analgesoidea parasites des oiseaux du Maroc
Injuries of terminal phalanges of the fingers in children
Biology of flowering and nectar production in pear (Pyrus communis)
Das Reliktvorkommen der Aspisviper (Vipera aspis L.) im Schwarzwald
Hydrological modelling of drained blanket peatland
Pathologic morphology and clinical significance of the anomalous origin of the left circumflex coronary artery from the right coronary artery. General review and autopsy analysis of 30 cases
Cyto genetic analyses of lymphocyte cultures after exposure to calcium cyclamate
Axelrodia riesei, a new characoid fish from Upper Rio Meta in Colombia With remarks concerning the genus Axelrodia and description of a similar, sympatric, Hyphessobrycon-species
Favorable evolution of a case of tuberculosis of pancreas under antibiotic action
RIFM fragrance ingredient safety assessment, Valencene, CAS Registry Number 4630-07-3
Parenteral microemulsions: an overview
Temperate pasture: management for grazing and conservation
Evaluation of a new coprocessed compound based on lactose and maize starch for tablet formulation
Thermal expansion and cracking of three confined water-saturated igneous rocks to 800C
Revision of the genera of the tribe Stigmoderini (Coleoptera: Buprestidae) a discussion of phylogenetic relationships
Anal tuberculosis. Report of a case
Gastric tuberculosis in the past and present
Adaptive responses of the cardiovascular system to prolonged spaceflight conditions: assessment with Holter monitoring

Immunofluorescence determination of IgG following high performance liquid gel permeation chromatography


Immunofluorescence determination of IgG following high performance liquid gel permeation chromatography



Journal of Immunological Methods 85(1): 115-119



ISSN/ISBN: 0022-1759

PMID: 3908558

DOI: 10.1016/0022-1759(85)90279-0

The application of high performance liquid gel permeation chromatography (HPL-GPC) and immunofluorescence for the estimation of a protein was studied using human IgG as a model system following separation of the fluorescent immune complex from free fluorescent-labelled antibody on a column of HPL-GPC. Using FITC-labelled anti-human IgG Fab fragment and HPL-GPC, nanogram levels of IgG could be determined within 5 min of mixing the antigen and antiserum. HPL-GPC immunofluorescence analysis is sensitive specific, simple, rapid and highly versatile.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 040359693

Download citation: RISBibTeXText

Related references

Immunofluorescence determination of immunoglobulin g following high performance liquid gel permeation chromatography. Journal of Immunological Methods 85(1): 115-120, 1985

Immunofluorescence determination of IgG in cerebrospinal fluid following high performance liquid gel permeation chromatography. Journal of Immunological Methods 98(1): 1-4, 1987

Determination of reactivity of aspartame with flavor aldehydes by gas chromatography high performance liquid chromatography and gel permeation chromatography. Journal of Food Science 49(2): 520-524, 1984

Determination of polycyclic aromatic hydrocarbons in shellfish and fish by gel permeation chromatography and high performance liquid chromatography. Alimentaria 40(Separata Al Numero 340): 3-14, 2003

Simultaneous determination of 14 oil-soluble synthetic dyes in chilli products by high performance liquid chromatography with a gel permeation chromatography clean-up procedure. Food Chemistry 145: 956-962, 2014

High-performance liquid chromatography determination of triptolide in vitro permeation studies. Analytica Chimica Acta 534(2): 215-221, 2005

Determination of N-methyl carbamate pesticides in diet samples by high performance liquid chromatography-linear ion trap mass spectrometry with gel permeation chromatography cleanup. Se Pu 32(5): 499-505, 2014

Determination of Polycyclic Aromatic Hydrocarbons in Camellia Oil by Gel Permeation and High-Performance Liquid Chromatography. Analytical Letters 48(14): 2172-2182, 2015

Determination by high-performance liquid chromatography of ketoprofen in vitro in rat skin permeation samples. Journal of Chromatography. a 870(1-2): 143-149, 2000

Determination of spinosad in vegetables and fruits by high-performance liquid chromatography with UV and mass spectrometric detection after gel permeation chromatography and solid-phase extraction cleanup on a 2-layered column. Journal of Aoac International 89(6): 1641-1649, 2006

Application of gel permeation chromatography and nonaqueous reverse phase chromatography to high performance liquid chromatographic determination of retinyl palmitate and alpha-tocopheryl acetate in infant formulas. Journal - Association of Official Analytical Chemists 65(4): 810-816, 1982

Determination of phthalate plasticizers in foods by high performance liquid chromatography with gel permeation chromatographic clean-up. Se Pu 29(12): 1236-1239, 2011

Simultaneous determination of physostigmine and tetrahydroaminoacridine in a transdermal permeation study by high-performance liquid chromatography. Journal of Chromatography 526(1): 87-95, 1990

Multiresidue determination of pesticides in plants by high-performance liquid chromatography following gel permeation chromatographic clean-up. Journal of Chromatography. a 823(1-2): 11-16, 1998

Determination of the integrity of human immunodeficiency virus gp120 by gel-permeation high performance liquid chromatography. FASEB Journal 7(7): A1253, 1993