+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Improved high-performance liquid chromatographic method for the determination of indomethacin in plasma



Improved high-performance liquid chromatographic method for the determination of indomethacin in plasma



Therapeutic Drug Monitoring 14(1): 61-65



Indomethacin is an inhibitor of prostaglandin synthesis and has several uses, including the ability to encourage closure of a patent ductus in premature neonates. A rapid, accurate, and sensitive high-performance liquid chromatographic (HPLC) method is described for measuring plasma concentrations of indomethacin. An acidic buffer (citrate, pH 3.0) was employed to alter the pH of the aqueous phase prior to extraction to minimise interference interference from endogenous compounds. Extraction of indomethacin from plasma was optimally achieved with petroleum ether (boiling fraction of 40-60 degrees C):dichloromethane (50:50). However, separation of indomethacin from plasma proteins was attempted unsuccessfully using acetonitrile precipitation; severe band broadening occurred due to injected sample solvent problems. The absolute recoveries for indomethacin and internal standard (mefenamic acid) were over 90% (n = 3). Precision was expressed as the coefficient of variation (n = 4), which was less than or equal to 8% at each of six indomethacin concentrations in the range 50-2,000 ng/ml. The limit of quantitation of indomethacin in plasma was 50 ng/ml (0.5 ml of plasma injected). We report an HPLC method for the quantitation of indomethacin in plasma that may have widespread applicability for routine drug assay laboratories.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 040380200

Download citation: RISBibTeXText

PMID: 1546391

DOI: 10.1097/00007691-199202000-00010


Related references

A fully automated high-performance liquid chromatographic method for the determination of indomethacin in plasma. Journal of Pharmaceutical and Biomedical Analysis 7(12): 1819-1827, 1989

Improved method for the determination of indomethacin in plasma by reversed phase high performance liquid chromatography. Farmaco, Edizione Pratica 41(9): 290-299, 1986

Improved high-performance liquid chromatographic method for the determination of quinine in plasma. Journal of Chromatography 430(1): 170-174, 1988

Improved high-performance liquid chromatographic method for the determination of coenzyme Q10 in plasma. Journal of Chromatography 593(1-2): 217-226, 1992

Improved method for the determination of indomethacin in plasma and urine by reversed-phase high-performance liquid chromatography. Journal of Chromatography 383(2): 432-437, 1986

Improved high performance liquid chromatographic method for the determination of 6 n n n trimethyllysine in plasma and urine biomedical application of chromatographic figures of merit and amine mobile phase modifiers. Journal of Chromatography Biomedical Applications 380(2): 285-300, 1986

An improved high performance liquid chromatographic method for determination of cimetidine and its major metabolite in plasma. Archivos de Investigacion Medica 20(4): 309-313, 1989

Quantitative determination of diclofenac, indomethacin, naproxen and their ester prodrugs in aqueous media and plasma by a simple high-performance liquid chromatographic method. Pharmaceutical Research (New York) 10(10 Suppl. ): S226, 1993

Improved high performance liquid chromatographic method for the determination of tris aminomethane in human plasma, erythrocytes, and whole blood. Journal of Liquid Chromatography & Related Technologies 21(11): 1701-1715, 1998

Improved high-performance liquid chromatographic method for the simultaneous determination of propranolol and 4-hydroxypropranolol in plasma with fluorescence detection. Journal of Chromatography 422: 294-300, 1987

Improved high-performance liquid chromatographic method to estimate aminosugars and its application to glycosaminoglycan determination in plasma and serum. Journal of Chromatography B 765(2): 151-160, 25 December, 2001

Improved high-performance liquid chromatographic method to estimate aminosugars and its application to glycosaminoglycan determination in plasma and serum. Journal of Chromatography. B Biomedical Sciences and Applications 765(2): 151-160, 2001

Improved high performance liquid chromatographic method for the determination of tris(hydroxymethyl)aminomethane (THAM) in human plasma, erythrocytes, and whole blood. Journal of Liquid Chromatography and Related Technologies 21(11): 1701-1715, 1998

Improved high-performance liquid chromatographic method for the simultaneous determination of caffeine and its N-demethylated metabolites in plasma using solid-phase extraction. Journal of Chromatography 342(1): 105-117, 1985