+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

In vitro calcium transport properties of skeletal muscle mitochondria from vitamin D-deficient and 1,25-dihydroxy-vitamin D3-treated chicks



In vitro calcium transport properties of skeletal muscle mitochondria from vitamin D-deficient and 1,25-dihydroxy-vitamin D3-treated chicks



Calcified Tissue International 47(1): 46-50



Previous work has shown that vitamin D3 or 1,25-dihydroxy-vitamin D3 affect calcium content and fluxes in mitochondria of chick skeletal muscle in situ. Studies were performed to investigate whether these effects are related to variations in the Ca2+ transport properties of mitochondrial membranes. Mitochondria isolated from skeletal muscle of vitamin D-deficient chicks and chicks dosed with 1,25(OH)2D3 for 3 or 7 days (50 ng/day) were employed. No changes in the rate and affinity for calcium of the Ruthenium Red-sensitive Ca2+ uptake system were detected after treatment with 1,25(OH)2D3. The metabolite did not cause either modifications in Ca2+ efflux from mitochondria preloaded with the cation induced by Na+ or blockage of mitochondria energy supply. Prior treatment of animals with vitamin D3 was also without effects. However, a significant stimulation of Ca2- uptake by intact muscle preparations from the same experimental animals was observed in response to treatment with 1,25(OH)2D3 in vivo (50 ng/day, 3 days) or in vitro (10(-10) M, 60 minutes). In addition, the Ca content of muscle mitochondria was markedly diminished in chicks treated with the sterol. It is suggested that the effects of 1,25(OH)2D3 on muscle mitochondrial Ca metabolism may be secondary to changes in cytoplasmic Ca2+.

Please choose payment method:






(PDF emailed within 1 workday: $29.90)

Accession: 040389437

Download citation: RISBibTeXText

PMID: 2369691


Related references

Skeletal muscle calcium metabolism and contractile force in vitamin D-deficient chicks. Journal of Clinical Investigation 64(5): 1157-1167, 1979

Suppression of 1,25-dihydroxy-vitamin D3-dependent calcium transport by protein synthesis inhibitors and changes in phospholipids in skeletal muscle. Biochimica et Biophysica Acta 845(2): 237-241, 1985

Effect of vitamin d 3 and 1 25 di hydroxy vitamin d 3 on calcium content in chicks skeletal muscle. Norman, A W Et Al (Ed ) Vitamin D: Chemical, Biochemical And Clinical Endocrinology Of Calcium Metabolism; Proceedings Of The 5th Workshop, Williamsburg, Va , Usa, Feb Xxxi+1288p Walter De Gruyter: Berlin, West Germany; New York, N Y , Usa Illus P1205-1208, 1982

In vitro cellular muscle calcium metabolism. Characterization of effects of 1,25-dihydroxy-vitamin D3 and 25-hydroxy-vitamin D3. Zeitschrift für Naturforschung. Section C Biosciences 40(1-2): 102-108, 1985

N tau methyl histidine release from skeletal muscle of vitamin d deficient rats treated with calcium or cholecalciferol. Federation Proceedings 41(5): ABSTRACT 7914, 1982

Ultrastructural changes in skeletal muscle of selenium-vitamin E-deficient chicks. American Journal of Veterinary Research 37(9): 1081-1089, 1976

Alterations in some biochemical constituents of skeletal muscle of vitamin E-deficient chicks. Journal of Nutrition 68(3): 359-369, 1959

Alterations in some Biochemical Constitutents of Skeletal Muscle of Vitamin E-Deficient Chicks. The Journal of Nutrition 68(3): 359-369, 1959

Comparison of sarcoplasmic reticulum calcium atpase from vitamin e deficient dystrophic rabbit skeletal muscle with iron ascorbate treated and untreated enzyme. Journal of Biochemistry (Tokyo) 103(2): 309-312, 1988

Calcium accumulation by intestinal mitochondria from rachitic and 1 25 di hydroxy vitamin d 3 treated chicks. Federation Proceedings 37(6): 1300, 1978

Biochemical and structural changes in skeletal muscle mitochondria in vitamin e deficient and re fed rabbits. Federation Proceedings 36(3): 1168, 1977

The effect of vitamin D3 and 1,25(OH)2D3 on Ca calcium content in chicks skeletal muscle. Vitamin D chemical biochemical and clinical endocrinology of calcium metabolism: proceedings of the Fifth Workshop on Vitamin D Williamsburg VA USA February 1982 editors AW Norman et al: -1207, 1982

Distribution of natural vitamin B12 a and of Co vitamin B12 in subcellular fractions of the liver of vitamin B12 deficient chicks. Comparative effects of vitamin B12 methionine and orotic acid. Italian Jour Biochem 9(6): 379-387, 1960

Distribution of natural vitamin B12 and of (58Co) vitamin B12 in subcellular fractions of the liver of vitamin B12-deficient chicks. Comparative effects of vitamin B12, methionine and orotic acid. Ital. J. Biochem 9: 379-387, 1960

Vitamin D3 and 1,25-dihydroxyvitamin D3 stimulate the skeletal muscle-calcium mobilization in rachitic chicks. Archives of Biochemistry and Biophysics 231(1): 211-216, 1984