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Chapter 40,390

In vitro construction of bacteriophage lambda and plasmid DNA molecules containing DNA fragments from bacteriophage T4

Velten, J.; Fukada, K.; Abelson, J.

Gene 1(1): 93-106

1976

ISSN/ISBN: 0378-1119
PMID: 802392
DOI: 10.1016/0378-1119(76)90009-3
Accession: 040389701
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Restriction endonucleases EcoRI and HindIII generated fragments of T4 cytosine-containing DNA were inserted into bacteriophage vector lambdagtSuIII and plasmid vectors pMB9 and pBR313. Resulting clones were screened for hybridization with 32P labeled T4 tRNA. Recombinant bacteriophages and plasmids were isolated which contained a T4 fragment coding for T4 RNA species 1 and 2 and T4 tRNA Arg. Selected lambda-T4 hybrid bacteriophages were grown to high titer and their DNA analyzed by gel electrophoresis.

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