+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

In vitro evaluation of a high-efficiency leukocyte adherence filter

In vitro evaluation of a high-efficiency leukocyte adherence filter

Annals of Hematology 63(6): 302-306

In view of the currently available data, prevention of alloimmunization requires filters with higher efficiency to achieve a reduction in the number of leukocytes below 10 million per transfusion. Two versions (Pall PL-100 and PL-50) of the new generation leukocyte-depletion filters were studied. Single donor (SDPC)- and pooled multiple donor (MDPC) platelets were run in parallel. At a flow rate of 10 ml/min, the PL-100 filter was shown to effectively reduce the number of residual leukocytes to far below the critical immunogenic threshold of 10 million in all SDPC units and in 77% of MDPC units. Apheresis platelets appear not only to be better depleted than pooled multiple donor platelets, but also to have a better post-filtration platelet recovery (96% versus 84%). The efficiency of the smaller version of the filter (Pall-50) was higher than that of the Pall-100 filter for both single and pooled multiple donor platelet concentrates (PC). Leukocytes were absent in more than 92% of units in both types of concentrates. The maximal number of detected leukocytes was 2.2 million in a pool of 6 units. The outcome of filtration of 5-day-old pooled platelets was less favorable than filtration of 1- or 2-day-old pooled platelets, indicating that filtration soon after preparation is preferred to filtration after storage. Post-filtration platelet integrity, activation state, function, and morphology were all well preserved in both single and multiple PCs.

Please choose payment method:

(PDF emailed within 0-6 h: $19.90)

Accession: 040390697

Download citation: RISBibTeXText

PMID: 1756191

DOI: 10.1007/bf01709651

Related references

The platelet/leukocyte ratio in red blood cell concentrates is an essential indicator of leukocyte removal filter efficiency which limits their use. Transfusion Clinique et Biologique 7(1): 70-75, 2000

Evaluation of the effect of media velocity on filter efficiency and most penetrating particle size of nuclear grade high-efficiency particulate air filters. Journal of Occupational and Environmental Hygiene 5(11): 713-720, 2008

In vitro biocompatibility evaluation of a stainless steel leukocyte filter: Cytokine production and protein adsorption. Japanese Journal of Artificial Organs 26(3): 613-617, 1997

Evaluation of a high-efficiency, filter-bank system. Journal of Occupational and Environmental Hygiene 3(4): 204, 2006

Correlation of extracts obtained by high efficiency gel chromatography of lactic dehydrogenase virus infected mouse serum and cytosol from human tumors using leukocyte adherence inhibition assay. Neoplasma 26(3): 261-266, 1979

In vitro evaluation of clot capture efficiency of an absorbable vena cava filter. Journal of Vascular Surgery. Venous and Lymphatic Disorders 4(4): 472-478, 2016

New optional IVC filter for percutaneous retrieval--in vitro evaluation of embolus capturing efficiency. Rofo 177(5): 632-636, 2005

Pre storage leukodepletion with a high efficiency filter in vitro parameters and in vivo red cell recovery. Transfusion 31(8 Suppl.): 18S, 1991

Combination of a simple hollow fiber filter system with a leukocyte filter for the preparation of leukocyte-depleted red cell concentrates and plasma. Kretschmer, V, Stangel, W, Wiebecke, D, Contributions to Infusion Therapy; Transfusion medicine 1991/92, Contributions to Infusion Therapy; Transfusionsmedizin 1991/92: 287-291, 1992

Residual leukocyte content of plateletpheresis concentrates produced on the haemonetics MCS+ Evaluation of a method to predict high post filter white cell counts. Transfusion 35(10 Suppl. ): 2S, 1995

Leukocyte adherence-related glycoproteins LFA-1, Mo1, and p150,95: a new group of monoclonal antibodies, a new disease, and a possible opportunity to understand the molecular basis of leukocyte adherence. Journal of Infectious Diseases 152(4): 661-664, 1985

Evaluation of vaporized hydrogen peroxide fumigation as a method for the bio-decontamination of the high efficiency particulate air filter unit. Biomedical and Environmental Sciences 26(2): 110-117, 2013

Development of a double-layered ceramic filter for aerosol filtration at high-temperatures: the filter collection efficiency. Journal of Hazardous Materials 136(3): 747-756, 2006

Regulation of leukocyte glass adherence and tube leukocyte adherence inhibition reactivity by serum factors in dogs with progressing or spontaneously regressing canine transmissible venereal sarcoma. Cancer Immunology Immunotherapy 19(3): 168-176, 1985

Regulation of leukocyte glass adherence and tube leukocyte adherence inhibition (LAI) reactivity by serum factors in dogs with progressing or spontaneously regressing canine transmissible venereal sarcoma (CTVS). Cancer Immunology ImmunoTherapy 19(3): 168-176, 1985