+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Induction of glial cell MHC antigen expression in neurotropic coronavirus infections. Characterization of the H-2-inducing soluble factor elaborated by infected brain cells



Induction of glial cell MHC antigen expression in neurotropic coronavirus infections. Characterization of the H-2-inducing soluble factor elaborated by infected brain cells



Journal of Immunology 140(6): 2068-2072



Neurotropic coronavirus (mouse hepatitis virus strain A59) infection induces major histocompatibility complex class I (H-2) surface antigens on oligodendrocytes and astrocytes, cells that do not normally express detectable MHC antigens on their surface. The induction on MHC antigen expression potentially allows immunocytes to interact with infected glial cells and may play a critical role in the development of virus-induced, immune-mediated demyelination in the central nervous system, a possible model of human multiple sclerosis. In this study, we characterized the soluble factor involved in MHC antigen induction, quantitated induction of MHC antigens, and analyzed the central nervous system cell type involved in the production of the factor. The H-2-inducing factor, most likely produced by astrocytes, was found to be nondialyzable, heat- and trypsin-sensitive, but resistant to treatment at pH 2.0. The m.w. of the factor was estimated as 50 to 100 kDa. Studies on fractionation by ultrafiltration and sucrose density gradient along with antibody-blocking experiments indicate that the factor is not interferon or virus particles.

Please choose payment method:






(PDF emailed within 1 workday: $29.90)

Accession: 040419875

Download citation: RISBibTeXText

PMID: 2831279


Related references

Soluble factors from murine coronavirus mhv a 59 infected glial cells induce h 2 antigen expression on mouse oligodendrocytes and astrocytes. Neurology 36(4 Suppl. 1): 313, 1986

Parallel induction of the formation of dopamine and its metabolites with induction of tyrosine hydroxylase expression in foetal rat and human cerebral cortical cells by brain-derived neurotrophic factor and glial-cell derived neurotrophic factor. Developmental Brain Research 127(2): 111-122, 30 April, 2001

Lowering corticosterone levels reinstates hippocampal brain-derived neurotropic factor and Trkb expression without influencing deficits in hypothalamic brain-derived neurotropic factor expression in leptin receptor-deficient mice. Neuroendocrinology 93(1): 58-64, 2011

Intervertebral disc degeneration-induced expression of pain-related molecules: glial cell-derived neurotropic factor as a key factor. Journal of Neurosurgical Anesthesiology 23(4): 329-334, 2011

Purification and initial characterization of a tumor-derived soluble factor inducing MMP-9 expression in a human monocyte cell line. Proceedings of the American Association for Cancer Research Annual Meeting 39: 293-294, 1998

Expression of glial cell line-derived neurotropic factor receptor alpha-1 in immature teratomas. American Journal of Clinical Pathology 130(6): 892-896, 2008

Soluble suppressor supernatants elaborated by concanavalin A-activated human mononuclear cells. I. Characterization of a soluble suppressor T cell proliferation. Journal of Immunology 126(3): 1185-1191, 1981

Soluble suppressor supernatants elaborated by concanavalin a activated human mononuclear cells 2. characterization of a soluble suppressor of b cell immuno globulin production. Journal of Immunology 126(3): 1192-1197, 1981

IFN increases class I MHC antigen expression on adenovirus-infected human cells without inducing resistance to natural killer cell killing. Journal of Immunology 149(7): 2372-2377, 1992

Cytokine-mediated induction of human immunodeficiency virus (HIV) expression and cell death in chronically infected U1 cells: do tumor necrosis factor alpha and gamma interferon selectively kill HIV-infected cells?. Journal of Virology 68(4): 2598-2604, 1994

Glial cell-line-derived neurotropic factor and its receptors are expressed by germinal and somatic cells of the rat testis. Journal of Endocrinology 190(1): 59-71, 2006

Bystander CD4 T cells do not mediate demyelination in mice infected with a neurotropic coronavirus. Journal of Neuroimmunology 137(1-2): 42-50, 2003

Characterization of blood mononuclear cells producing IFN alpha following induction by coronavirus-infected cells (porcine transmissible gastroenteritis virus). Research in Immunology 141(2): 141-151, 1990

Intrathecal granulocyte colony-stimulating factor modulate glial cell line-derived neurotrophic factor and vascular endothelial growth factor A expression in glial cells after experimental spinal cord ischemia. Neuroscience 242: 39-52, 2013

Viral expression of CCL2 is sufficient to induce demyelination in RAG1-/- mice infected with a neurotropic coronavirus. Journal of Virology 79(11): 7113-7120, 2005