+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Inhibition of alkaline phosphatase activity and D-glucose uptake in rat renal brush-border membrane vesicles by aminoglycosides



Inhibition of alkaline phosphatase activity and D-glucose uptake in rat renal brush-border membrane vesicles by aminoglycosides



Biochimica et Biophysica Acta 903(1): 31-36



The binding of aminoglycoside antibiotics to, and their effects on, the plasma membrane were studied using isolated rat renal brush-border membrane vesicles. Dibekacin was noted to bind with brush-border membrane vesicles having a single class of many binding sites. 3H-labeled dibekacin binding was inhibited competitively by unlabeled dibekacin, gentamicin or amikacin. The inhibition constants obtained from the Dixon plots followed the order of gentamicin approximately equal to dibekacin greater than amikacin. The alkaline phosphatase activity of brush-border membrane vesicles was inhibited by gentamicin significantly, as was also observed by a histochemical study. Sodium-dependent D-glucose uptake by brush-border membrane vesicles was significantly inhibited by the addition of gentamicin.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 040443879

Download citation: RISBibTeXText

PMID: 3651455

DOI: 10.1016/0005-2736(87)90152-0


Related references

Membrane alkaline phosphatase activity and phosphate transport in mouse renal brush border membrane vesicles. Kidney International 16(6): 840, 1979

Cyclosporine inhibits phosphate transport and stimulates alkaline phosphatase activity in renal brush border membrane vesicles. FASEB Journal 5(4): A762, 1991

Role of protein thiols in inhibition of sodium-coupled glucose uptake by cisplatin in renal brush-border membrane vesicles. Journal of Pharmacology and Experimental Therapeutics 284(1): 142-150, 1998

Correlation between alkaline phosphatase and phosphate transport in rat renal brush border membrane vesicles. Pfluegers Archiv European Journal of Physiology 384(2): 149-154, 1980

On the correlation between alkaline phosphatase and phosphate transport in rat renal brush border membrane vesicles. Pflugers Archiv 384(2): 149-153, 1980

Role of alkaline phosphatase in phosphate uptake into brush border membrane vesicles from human intestinal mucosa. Journal of Biochemistry 97(5): 1461-1466, 1985

Inhibition of d glucose uptake by sorbitol in jejunal brush border membrane vesicles. Gastroenterology 100(5 Part 2): A696, 1991

Selective removal of alkaline phosphatase from renal brush border membrane and sodium dependent brush border membrane transport. Journal of Biological Chemistry 258(9): 5695-5701, 1983

Zinc inhibition of glucose uptake in brush border membrane vesicles from pig small intestine. Pfluegers Archiv European Journal Of Physiology. 415(2): 165-171, 1989

Effect of anti tubule basement membrane antibody on glucose uptake by rat renal brush border membrane vesicles. Kidney International 23(1): 182, 1983

Myo inositol transport in renal brush border membrane vesicles and its inhibition by d glucose. Renal Physiology 2(3): 160, 1979

Zinc inhibition of sodium stimulated glucose uptake by intestinal brush border membrane vesicles. Federation Proceedings: 1081, 1987

Potential-dependent D-glucose uptake by renal brush border membrane vesicles in the absence of sodium. American Journal of Physiology 242(4): F340-F345, 1982

Lead nitrate inhibits glucose and alanine uptake by rat renal brush border membrane vesicles. Clinical Research 34(2): 589A, 1986

Interactions between Na+-dependent uptake of D-glucose, phosphate and L-alanine in rat renal brush border membrane vesicles. Biochimica et Biophysica Acta 647(2): 203-210, 1981