+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Inhibition of oleandomycin biosynthesis by glucose



Inhibition of oleandomycin biosynthesis by glucose



Antibiotiki i Meditsinskaia Biotekhnologiia 30(8): 579-584



The results of the study on the effect of glucose and various carbohydrates on biosynthesis of oleandomycin by Streptomyces antibioticus are presented. It was found that glucose added at the beginning or by the 48th hour of the fermentation process on the complex medium inhibited oleandomycin biosynthesis. To investigate the mechanism of the glucose effect, a fermentation medium was developed. It provided variation of the carbohydrate composition, determination of the protein content in the culture and evaluation of the mycelium productivity. With the use of this medium it was shown that monosaccharides such as galactose, fructose and glucose significantly activated the mycelium growth as compared to lactose and sucrose. At the same time glucose completely inhibited oleandomycin biosynthesis when added either as an only carbohydrate component or in combination with galactose or fructose, while the presence of the other two monosaccharides did not prevent antibiotic production, though the mycelium productivity was lowered as compared to that with the use of the disaccharide. Therefore, the inhibitory effect of glucose on biosynthesis of oleandomycin was not connected with activation of the culture growth by it. Acidification of the medium on cultivation of the streptomycete in the presence of glucose only partially explained its inhibitory effect, since inhibition was maintained on the medium with addition of CaCO3 which stabilizes pH. Addition of 2-deoxy-D-glucose, a nonmetabolized glucose analog, to the fermentation medium retarded antibiotic production. It is possible that the inhibitory effect of glucose on biosynthesis of oleandomycin is not associated with its metabolism.

Please choose payment method:






(PDF emailed within 1 workday: $29.90)

Accession: 040447551

Download citation: RISBibTeXText

PMID: 3904619


Related references

Glucose effect on oleandomycin biosynthesis by actinomyces antibioticus. Szabo, G , S Biro And M Goodfellow (Ed ) Symposia Biologica Hungarica, Vol 32 Biological, Biochemical And Biomedical Aspects Of Actinomycetes, Parts A And B; Sixth International Symposium on Actinomycetes Biology, Debrecen, Hungary, August 26-30, 1985 Xxviii+429p (Part A); Xxii+454p (Part B) Akademiai Kiado: Budapest, Hungary Illus 307, 1986, 1987

Repression of oleandomycin biosynthesis by glucose added in the process of fermentation. Mikrobiologiya 56(4): 593-599, 1987

Biosynthesis of the macrolide oleandomycin by Streptomyces antibioticus. Purification and kinetic characterization of an oleandomycin glucosyltransferase. Journal of Biological Chemistry 270(31): 18234-9, 1995

Inhibition of oleandomycin synthesis by glucose. Antibiotiki i Meditsinskaya Biotekhnologiya 30(8): 579-584, 1985

Oleandomycin and its natural and synthetic analogs. II. Structure and properties of oleandomycin B, a new compound of the oleandomycin group. Antibiotiki i Khimioterapiia 34(3): 199-202, 1989

Transfer of glucose in the biosynthesis of thyroid glyco proteins 1. inhibition of glucose transfer to oligo saccharide lipids by gdp mannose. Biochimica et Biophysica Acta 674(1): 48-57, 1981

Effect of certain factors on the biosynthesis of oleandomycin. Antibiotiki 7: 957-962, 1962

Synthetic medium for oleandomycin biosynthesis. Antibiotiki 10(6): 502-507, 1965

Effect of phosphorus on the biosynthesis of oleandomycin. Antibiotiki 11(3): 205-210, 1966

Scaling conditions of oleandomycin biosynthesis. Antibiotiki 29(4): 243-245, 1984

Transfer of glucose in the biosynthesis of thyroid glycoproperties I. Inhibition of glucose transfer to oligosaccharide lipids by GDP-mannose. Biochimica et Biophysica Acta - General Subjects 674(1): 48-57, 1981

Respiration of Actinomyces antibioticus during the biosynthesis of oleandomycin. Mikrobiologiia 41(1): 44-47, 1972

Conditions for grading the process of oleandomycin biosynthesis. Antibiotiki 29(4): 243-245, 1984

Effect of conditions of agitation and aeration on the biosynthesis of oleandomycin. Antibiotiki 28(11): 806-810, 1983

Study of the growth patterns of Act. antibioticus and the biosynthesis of oleandomycin. Antibiotiki 21(3): 195-200, 1976