+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Interference by human anti-mouse antibodies in CA 125 assay after immunoscintigraphy: anti-idiotypic antibodies not neutralized by mouse IgG but removed by chromatography

Interference by human anti-mouse antibodies in CA 125 assay after immunoscintigraphy: anti-idiotypic antibodies not neutralized by mouse IgG but removed by chromatography

Clinical Chemistry 36(7): 1333-1338

Falsely increased concentrations of the ovarian carcinoma-associated antigen, CA 125, were measured by a monoclonal antibody (MAb)-based double determinant immunoradiometric assay (IRMA) in patients who developed antibodies to mouse immunoglobulins (IgGs) after receiving injections of the same MAb as is used in the CA 125 IRMA. Addition of undiluted mouse serum or purified mouse IgG to the assay mixture failed to eliminate the falsely increased CA 125 concentrations in most of the samples, owing to the presence of anti-idiotype antibody. Because of their anti-idiotypic nature, the human anti-mouse antibodies (HAMAS) had only little effect on other immunometric assays, and this effect could be completely eliminated by addition of mouse IgG. To eliminate the effect of HAMA on the CA 125 assay, we studied the ability of various chromatographic methods to separate the interfering HAMA from CA 125. For measuring HAMA in serum and chromatographic fractions we developed a time-resolved fluoroimmunoassay. Adequate separation of CA 125 and HAMA was achieved by affinity chromatography of patients' sera with solid-phase Protein A, Protein G, cation-exchange chromatography on Mono S, and gel filtration on Superose 6. These results demonstrate that the interference can effectively be removed by rather simple chromatographic procedures.

Please choose payment method:

(PDF emailed within 1 workday: $29.90)

Accession: 040471384

Download citation: RISBibTeXText

PMID: 2372947

Related references

Development of anti mouse immunoglobulin g anti idiotypic antibodies by patients receiving radiolabeled monoclonal antibody 791t 36 for diagnostic immunoscintigraphy. British Journal of Cancer 52(3): 447, 1985

Scintigraphic detection of infection: are human anti-mouse antibodies a relevant problem in immunoscintigraphy with anti-granulocyte antibodies?. Nuclear Medicine Communications 16(2): 63-65, 1995

Epitope complementarity and idiotypic interactions: a study of idiotypic-like interactions between anti-cytidine and anti-guanosine A/J mouse monoclonal antibodies--II. Analysis of the gene repertoire used to encode the variable regions of these antibodies. Molecular Immunology 31(1): 75-76, 1994

Human anti mouse response after immunoscintigraphy is with chimeric ch anti cea monoclonal antibodies mab. Journal of Nuclear Medicine 33(5 Suppl.): 903-904, 1992

Induction of anti idiotypic ab 2 and anti anti idiotypic ab 3 antibodies in patients treated with the mouse monoclonal antibody 17 1a ab 1 relation to the clinical outcome an important antitumoral effector function. Hybridoma 10(4): 421-432, 1991

Induction of Anti-Idiotypic (ab 2 ) and Anti-Anti-Idiotypic (ab 3 ) Antibodies in Patients Treated with the Mouse Monoclonal Antibody 17-1A (ab 1 ). Relation to the Clinical Outcome An Important Antitumoral Effector Function?. Hybridoma 10(4): 421-431, 1991

Human high molecular weight-melanoma associated antigen mimicry by mouse anti-idiotypic MAb MK2-23: Immunohistochemical analysis of the reactivity of anti-anti-idiotypic MAb with surgically removed melanoma lesions. International Journal of Cancer 60(3): 334-340, 1995

Induction of anti-DNP antibodies: suppressive effect of circulating anti-idiotypic antibodies to mouse myeloma protein MOPC 315. Journal of Immunology 113(1): 416-420, 1974

Induction of anti di nitro phenyl antibodies suppressive effect of circulating anti idiotypic antibodies to mouse myeloma protein mopc 315. Journal of Immunology 113(1): 417-420, 1974

Chicken antibodies: a tool to avoid interference by human anti-mouse antibodies in ELISA after in vivo treatment with murine monoclonal antibodies. Hybridoma 11(1): 33-39, 1992

Activating anti-idiotypic human anti-mouse antibodies for immunotherapy of ovarian carcinoma. Cancer (philadelphia). 73(3 Suppl.): 1121-1125, 1994

Structural studies on induced antibodies with defined idiotypic specificities determination of the variable region heavy chain subgroup of mouse anti ars and anti streptococcal antibodies. Immunogenetics 4(5): 423-424, 1977

Quantitation of IgG and IgM human anti-mouse antibodies (HAMA) interference in CA 125 measurements using affinity chromatography. Clinical Chemistry and Laboratory Medicine 36(1): 23-28, 1998

Anti-immunoglobulin antibodies IV. Cross-reaction of anti-idiotypic antibodies specific for rabbit and murine anti-a1 allotype antibodies with Fc fragment of human immunoglobulins. European Journal of Immunology 14(6): 548-552, 1984

Syngeneic anti-idiotypic monoclonal antibodies against on immunosuppressive mouse anti-mouse T cell mAb. Immunobiology 189(1-2): 197-198, 1993