+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Interleukin-8 is a major neutrophil chemotactic factor derived from cultured human gingival fibroblasts stimulated with interleukin-1 beta or tumor necrosis factor alpha



Interleukin-8 is a major neutrophil chemotactic factor derived from cultured human gingival fibroblasts stimulated with interleukin-1 beta or tumor necrosis factor alpha



Infection and Immunity 60(12): 5253-5258



Inflammatory mediators produced by cells in the gingiva have been implicated in the initiation and progression of periodontal disease, a common infectious disease. In this study, we examined the biological activity of neutrophil chemotactic factors and the kinetics of expression of interleukin-8 (IL-8) mRNA derived from normal gingival fibroblasts in response to inflammatory mediators in an in vitro model. Gingival fibroblasts stimulated by either recombinant human interleukin-1 beta or recombinant human tumor necrosis factor alpha produced neutrophil chemotactic factors after 4 h, whereas expression of cell-derived IL-8 mRNA was detected within 1 h after stimulation. Furthermore, in a neutralization assay, rabbit anti-recombinant human IL-8 antiserum inhibited neutrophil chemotactic activity to basal levels. These results provide evidence that gingival fibroblasts synthesize potent chemotactic factors such as IL-8 in the presence of the inflammatory mediators interleukin-1 beta and tumor necrosis factor alpha. The activity of these factors may contribute to neutrophil-mediated processes in the pathogenesis of periodontal disease.

Please choose payment method:






(PDF emailed within 1 workday: $29.90)

Accession: 040475127

Download citation: RISBibTeXText

PMID: 1452358


Related references

Prostaglandin E2 induced abortion with vaginal suppositories in a contraceptive diaphragm. Prostaglandins 7(3): 195-205, 1974

Synergistic effect of interleukin-1-beta and tumor necrosis factor alpha on interleukin-8 gene expression in synovial fibroblasts Evidence that interleukin-8 is the major neutrophil-activating chemokine released in response to monokine activation. Arthritis and Rheumatism. 36(9): 1295-1304, 1993

Synergistic effect of interleukin-1 beta and tumor necrosis factor alpha on PGE2 production by articular chondrocytes does not involve PLA2 stimulation. Experimental Cell Research 222(2): 379-384, 1996

Production of interleukin-11 and interleukin-6 in cultured human gingival fibroblasts with the stimulation of lipopolysaccharides. Zhonghua Kou Qiang Yi Xue Za Zhi 42(1): 34-36, 2007

Dual regulatory action of prostatic inhibin (10.7 kDa) on DNA synthesis. Indian Journal of Experimental Biology 30(11): 1024-1029, 1992

Stimulation of the human immunodeficiency virus type 2 hiv 2 gene expression by the cytomegalovirus and hiv 2 transactivator gene. AIDS Research and Human Retroviruses 6(5): 649-658, 1990

Bradykinin receptors in the zebrafish (Danio rerio). Annals of the new York Academy of Sciences 1040: 246-248, 2005

The intravascular mass of albumin during human pregnancy: A serial study in normal and diabetic women. British Journal of Obstetrics and Gynaecology 100(6): 587-592, 1993

Effect of triclosan on interferon-gamma production and major histocompatibility complex class II expression in human gingival fibroblasts. Journal of Clinical Periodontology 27(10): 733-737, 2000

Influence of carbon monoxide on the colour stability of defrosted yellowfin tuna (Thunnus albacares) muscle stored under aerobic and anaerobic conditions. Journal of the Science of Food and Agriculture 95(8): 1605-1612, 2015

Production of interleukin-1 alpha and interleukin-2 by mononuclear cells from children with growth delay in relation to the degree of growth hormone deficiency: effects of substitutive treatment. Hormone Research 34(5-6): 209-214, 1990

Effects of interleukin (IL)-6 on luteinizing hormone- and IL-1beta-induced ovulation and steroidogenesis in the rat ovary. Biology of Reproduction 58(5): 1266-1271, 1998

Effect of phenytoin on cytochrome P450 2B mRNA expression in primary rat astrocyte cultures. Journal of Neuroscience Research 54(3): 402-411, 1998

Benzydamine protection in a mouse model of endotoxemia. Inflammation Research 46(9): 332-335, 1997

Interleukin-1-beta and tumor necrosis factor alpha signal through structurally distinct phosphatidic acid molecules in human umbilical vein endothelial cells. Clinical Research 42(2): 135A, 1994