+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Intracisternal administration of Mycobacterium tuberculosis potentiates the delayed type hypersensitivity in guinea-pigs

Intracisternal administration of Mycobacterium tuberculosis potentiates the delayed type hypersensitivity in guinea-pigs

Physiologia Bohemoslovaca 39(3): 193-198

The delayed type hypersensitivity (DTH) was studied in guinea-pigs using the skin test. The mycobacterium tuberculosis (M. tbc)--was applied by various routes. The control group received ovalbumin in Freund's incomplete adjuvant (FIA) into the footpad. The first experimental group received ovalbumin in Freund's complete adjuvant (FCA) into the footpad. The other experimental groups always received, in addition to ovalbumin plus FIA into the footpad, the M. tbc. 1. intracisternally, 2. intramuscularly, 3. intraperitoneally, 4. orally. On the day of administration of the sensibilizing substance, the body temperature was monitored. The skin test was measured after 14 and 21 days. It was established that, for the study of the DTH, the 21-day interval was more significant than the 14-day interval. A 100 times smaller dose of M. tbc. given intracisternally had the same immunostimulating effect as the injection of ovalbumin with M. tbc into the footpad (p less than 0.01). The size of the skin reaction was not only significantly influenced by the intramuscular and oral administration of M. tbc. On the other hand, the intraperitoneal administration inhibited the DTH (p less than 0.01). The increase of body temperature after the administration of M. tbc. correlated with the influence on the DTH except for the intraperitoneal administration. The route of the M. tbc. administration was crucial for the development of the DTH.

Please choose payment method:

(PDF emailed within 1 workday: $29.90)

Accession: 040487526

Download citation: RISBibTeXText

PMID: 2145596

Related references

Delayed-type hypersensitivity to a recombinant mycobacterial antigen, MPB64, in guinea pigs sensitized to Mycobacterium tuberculosis or Mycobacterium bovis BCG. Journal of Leukocyte Biology 57(2): 221-225, 1995

MTSA-10, the product of the Rv3874 gene of Mycobacterium tuberculosis, elicits tuberculosis-specific, delayed-type hypersensitivity in guinea pigs. Infection and Immunity 68(2): 990-993, 2000

Delayed type hypersensitivity responses to recombinant mycobacterial antigen 38,000 in guinea pigs sensitized to Mycobacterium tuberculosis. Zhonghua Jie He He Hu Xi Za Zhi 23(3): 161-163, 2002

Recombinant Rv3619c, Rv3872, Rv3873, Rv3874 and Rv3875 proteins of Mycobacterium tuberculosis induce delayed type hypersensitivity responses in guinea pigs. Abstracts of the General Meeting of the American Society for 106): 169, 2006

Delayed type skin reactions in guinea pigs sensitized with various acid fast bacilli and tested with protoplasms from homologous and heterologous organisms mycobacterium tuberculosis mycobacterium balnei mycobacterium batteyi mycobacterium bovis mycobacterium kansasii. International Archives Of Allergy & Applied Immunology: 283-292, 1968

Delayed-type hypersensitivity responses to ESAT-6 and MPT64 from Mycobacterium tuberculosis in the guinea pig. Infection and Immunity 66(7): 3454-3456, 1998

Delayed hypersensitivity reaction to tuberculin in mice and guinea pigs infected with Mycobacterium tuberculosis and treated with rifabutin or rifampicin. IXTH INTERNATIONAL CONFERENCE ON AIDS AND THE IVTH STD WORLD CONGRESS [Author] IXth International Conference on AIDS in affiliation with the IVth STD World Congress : 332, 1993

Delayed-type hypersensitivity and immunity against aerogenic tuberculosis in guinea pigs. Infection and Immunity 9(5): 815-820, 1974

Evaluation of Mycobacterium tuberculosis specific RD antigens for delayed type hypersensitivity responses in guinea pig. Indian Journal of Experimental Biology 48(2): 117-123, 2010

Effect of cyclopiazonic acid on delayed hypersensitivity to Mycobacterium tuberculosis, complement activity, serum enzymes, and bilirubin in guinea pigs. Mycopathologia 96(2): 73-77, 1986

The influence of the way of bcg administration in guinea pigs on the degree of delayed type hypersensitivity. International Association Of Biological Standardization Developments in Biological Standardization, Vol 58 Parts A And B Bcg Vaccines And Tuberculins; Symposium, Budapest, Hungary, September 6-9, 1983 Xviii+474p (Part A); Xviii+306p (Part B) S Karger Ag: Basel, Switzerland; New York, New York, Usa Illus Paper 563-564, 1986, 1987

Delayed hypersensitivity responses in mice and guinea pigs to Mycobacterium leprae, Mycobacterium vaccae, and Mycobacterium nonchromogenicum cytoplasmic proteins. Infection and Immunity 25(1): 229-236, 1979

Use of synthetic peptides corresponding to sequences of Mycobacterium leprae proteins to study delayed-type hypersensitivity response in sensitized guinea pigs. International Journal of Leprosy and Other Mycobacterial Diseases 60(1): 18-27, 1992

Fate of Mycobacterium tuberculosis & M. scrofulaceum in guinea pigs after intracisternal inoculation. Indian Journal of Medical Research 78: 751-755, 1983

Delayed hypersensitivity in vitro. 1. Inhibition of the antigen against migration of cells in the spleen and lymph nodes of guinea pigs sensitized to delayed-type hypersensitivity. Igaku to Seibutsugaku. Medicine and Biology 77(4): 123-129, 1968