+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Investigations on isolated islets of langerhans in vitro. 16.Modification of the glucose-dependent inhibition of glucagon secretion



Investigations on isolated islets of langerhans in vitro. 16.Modification of the glucose-dependent inhibition of glucagon secretion



Biochimica et Biophysica Acta 499(3): 362-372



Investigation of glucagon secretion in isolated Wistar rat islets was carried out to elucidate further the regulatory function of glucose and arginine on pancreatic A-cells. The suppressive effect of D-glucose could also be demonstrated with L-glucose, D-mannose, D-fructose, D-galactose, D-glyceraldehyde and DL-dihydroxyacetone, but not in the presence of 3-O-methylglucose or mannitol. Sugars other than D-glucose inhibited glucagon secretion only at much higher concentrations than those at which D-glucose was effective. Furthermore, although 7.5 mM D-glucose up to 80% inhibition, the effects of other sugars appeared to level off at only 50--60% inhibition. The inhibitory action of D-glucose or D-glyceraldedyde on glucagon secretion could not be overcome by L-arginine, but 3-O-methylglucose, mannoheptulose, 2-deoxy-D-glucose, iodoacetamide, theophylline, epinephrine and acetylcholine were effective. The insulin secretion in response to glucose was inhibited by the metabolic inhibitors used, whereas the B-cell response in the presence of glyceraldehyde was diminished by iodoacetamide only. Like D-glucose, a variety of other sugars markedly reduced the stimulatory effect of L-arginine in glucagon release. The results show that the suppression of glucagon secretion is not specific for D-glucose and not strongly connected on a stimulated insulin secretion.

Please choose payment method:






(PDF emailed within 1 workday: $29.90)

Accession: 040502239

Download citation: RISBibTeXText

PMID: 334269


Related references

Investigations on isolated islets of Langerhans in vitro. XIV. Insulin secretion and insulin stores of cultivated islets from sand rats (Psammomys obesus): investigations of glucose-dose response. Endokrinologie 68(1): 95, 1976

Investigations on isolated Islets of Langerhans in vitro. 14. Insulin secretion and insulin stores of cultivated islets from sand rats (Psammomys obesus): investigations of glucose-dose response. Endokrinologie 681: 95-103, 1976

Effect of some glucose transport inhibitors of the insulin and glucagon secretion in isolated islets of langerhans. Diabetologia 12(4): 412, 1976

Investigations on islets of langerhans in vitro. VIII. Ultrastructure and insulin secretion of isolated rat islets after different digestion with collagenase. Acta Histochemica 51(1): 50-60, 1974

Mechanism of halothane inhibition of glucose stimulated insulin secretion from isolated rat islets of langerhans. Federation Proceedings 33(3 Part 1): 275, 1974

Inhibition of glucagon secretion by glucose and glyceraldehyde on isolated islets of Wistar rats. Febs Letters 49(1): 100-102, 1974

Insulin secretagogues with an imidazoline structure inhibit arginine-induced secretion from isolated glucagon secretion from isolated rat islets of Langerhans. Biochemical and Biophysical Research Communications 236(1): 162-166, 1997

Insulin and glucagon secretion by isolated islets of Langerhans. Archives Internationales de Physiologie et de Biochimie 77(3): 531-532, 1969

Studies on glucagon secretion using isolated islets of Langerhans of the rat. Diabetologia 12(4): 301-306, 1976

Glucose metabolism of isolated mammalian islets of Langerhans; effects of glucagon, 2-deoxy-D-glucose and D-mannoheptulose. Diabetologia 4(5): 249-252, 1968

Investigations on isolated islets in vitro. 9. Influence of food deprivation and glucose refeeding in vitro on insulin secretion. Acta Diabetologica Latina 11(6): 457-467, 1974

Effects of fasting on insulin and glucagon secretion by isolated rat islets of Langerhans. Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine 154(2): 210-214, 1977

Role of second messengers in the regulation of glucagon secretion from isolated rat islets of Langerhans. Molecular and Cellular Endocrinology 50(1-2): 37-44, 1987

Investigations on isolated islets in vitro part 9 influence of food deprivation and glucose re feeding in vitro on insulin secretion. Acta Diabetologica Latina 11(6): 457-465, 1974

Enhancement of glucagon secretion from isolated rat islets of Langerhans by phorbol 12-myristate 13-acetate. Biochemical Journal 233(1): 287-289, 1986