+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Isolation and nucleotide sequence of rat Cu/Zn superoxide dismutase cDNA clones

Isolation and nucleotide sequence of rat Cu/Zn superoxide dismutase cDNA clones

Free Radical Research Communications 3(6): 337-346

Superoxide dismutase (SOD: EC catalyzes the dismutation of oxygen radicals and is thought to protect cells against free radical damage. We have isolated and sequenced cDNA clones of the rat Cu/Zn SOD, and have used these clones to map the rat genomic sequences coding for this enzyme. Rat Cu/Zn SOD is coded for by a single copy gene which is transcribed into an mRNA species of approximately 800 bases. Comparison of the nucleotide sequences of rat and human SOD cDNAs shows that they are homologous over 83% of the coding sequences and that in the 3'-untranslated region the extent of homology drops to 66%. The predicted rat SOD amino acid sequence is very similar to that of other eukaryotic SODs, showing 70% homology with the SODs of other mammals. Sequence conservation is particularly high in domains believed to be of functional importance.

Please choose payment method:

(PDF emailed within 0-6 h: $19.90)

Accession: 040519866

Download citation: RISBibTeXText

PMID: 3508449

DOI: 10.3109/10715768709088074

Related references

Isolation of two cDNA clones from tomato containing two different superoxide dismutase sequences. Plant Molecular Biology 11(5): 609-623, 1988

Nucleotide sequence of cDNA for the cytosolic Cu/Zn-superoxide dismutase from spinach (Spinacia oleracea L.). Nucleic Acids Research 18(16): 4923-4923, 1990

Isolation and sequence analysis of a cDNA that encodes pea manganese superoxide dismutase. Plant Molecular Biology 17(6): 1271-1274, 1991

Human Cu/Zn superoxide dismutase cDNA: isolation of clones synthesising high levels of active or inactive enzyme from an expression library. Nucleic Acids Research 13(6): 2017-2034, 1985

Manganese superoxide dismutase: nucleotide and deduced amino acid sequence of a cDNA encoding a new human transcript. Biochimica et Biophysica Acta 1087(2): 250-252, 1990

Nucleotide sequence of bovine copper/zinc superoxide dismutase cDNA generated by the polymerase chain reaction. Nucleic Acids Research 18(23): 7171-7171, 1990

Isolation and analysis of partial cDNA sequence coding for superoxide dismutase in Wuchereria bancrofti. Infection Genetics and Evolution 6(4): 287-291, 2006

Rat lung Cu,Zn superoxide dismutase. Isolation and sequence of a full-length cDNA and studies of enzyme induction. Journal of Clinical Investigation 83(4): 1241-1246, 1989

Nucleotide sequence of Streptococcus mutans superoxide dismutase gene and isolation of insertion mutants. Journal of Bacteriology 174(15): 4928-4934, 1992

Isolation and nucleotide sequence of cecropin B cDNA clones from the silkworm, Bombyx mori. Biochimica Et Biophysica Acta 1132(2): 203-206, 1992

Isolation and nucleotide sequence of cDNA clones encoding potato chitinase genes. Plant Molecular Biology 13(2): 249-250, 1989