+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Lithium ion inhibits nerve growth factor-induced neurite outgrowth and phosphorylation of nerve growth factor-modulated microtubule-associated proteins

Lithium ion inhibits nerve growth factor-induced neurite outgrowth and phosphorylation of nerve growth factor-modulated microtubule-associated proteins

Journal of Cell Biology 101(3): 862-870

LiCl (2.5-20 mM) reversibly suppressed nerve growth factor (NGF)-induced neurite outgrowth by cultured rat PC 12 pheochromocytoma cells. Similar concentrations of LiCl also reversibly blocked NGF-dependent regeneration of neurites by PC12 cells that had been primed by long-term pre-exposure to NGF and by cultured newborn mouse sympathetic neurons. In contrast, transcription-dependent responses of PC12 cells to NGF such as priming and induction of the NGF-inducible large external glycoprotein, occurred despite the presence of Li+. SDS PAGE analysis of total cellular phosphoproteins (labeled by 2-h exposure to 32P-orthophosphate) from neurite-bearing primed PC12 cells revealed that Li+ reversibly inhibited the phosphorylation of a band of Mr 64,000 that was barely detectable in NGF-untreated PC12 cells. However, Li+ did not appear to affect the labeling of other phosphoproteins in either NGF-primed or untreated PC12 cultures, nor did it affect the rapid increase in phosphorylation of several proteins that occurs when NGF is first added to unprimed cultures. Several criteria indicated that the NGF-inducible phosphoprotein of Mr 64,000 is a microtubule-associated protein (MAP). Of the NGF-inducible phosphorylated MAPs that have been detected in PC12 cells (Mr 64,000, 72,000, 80,000, and 320,000), several (Mr 64,000, 72,000, and 80,000) were found to be substantially less phosphorylated in the presence of Li+. Neither a phorbol ester tumor promotor nor permeant cAMP analogs reversed the inhibitory effects of Li+ on neurite outgrowth or on phosphorylation of the component of Mr 64,000. Microtubules are a major and required constituent of neurites, and MAPs may regulate the assembly and stability of neuritic microtubules. The observation that Li+ selectively inhibits NGF-induced neurite outgrowth and MAP phosphorylation suggests a possible causal relationship between these two events.

Please choose payment method:

(PDF emailed within 0-6 h: $19.90)

Accession: 040584318

Download citation: RISBibTeXText

PMID: 4030895

DOI: 10.2307/1611530

Related references

Lithium inhibits nerve growth factor induced neurite outgrowth and phosphorylation of certain nerve growth factor responsive microtubule associated phosphoproteins. Federation Proceedings 44(3): 413, 1985

Lithium ion blocks nerve growth factor induced neurite outgrowth but is permissive for nerve growth factor induced priming. Federation Proceedings 43(4): ABSTRACT 3460, 1984

PC12 cells express juvenile microtubule-associated proteins during nerve growth factor-induced neurite outgrowth. Journal of Cell Biology 107(2): 643-650, 1988

Microinjection of a p21ras antibody into PC12 cells inhibits neurite outgrowth induced by nerve growth factor and basic fibroblast growth factor. Growth Factors 4(2): 145-155, 1991

K-252a, a potent protein kinase inhibitor, blocks nerve growth factor-induced neurite outgrowth and changes in the phosphorylation of proteins in PC12h cells. Journal of Cell Biology 107(4): 1531-1539, 1988

Changes in the phosphorylation and distribution of vinculin during nerve growth factor induced neurite outgrowth. Developmental Biology 121(1): 97-104, 1987

Pressure microinjection of nerve growth factor and anti-nerve growth factor into the nucleus and cytoplasm: lack of effects on neurite outgrowth from pheochromocytoma cells. Journal of Neuroscience 3(7): 1488-1494, 1983

Nerve growth factor promotes cell survival and neurite outgrowth of rat central nervous system neurons in culture correlation with ontogenesis of nerve growth factor receptor immunoreactivity. Society for Neuroscience Abstracts 12(2): 1093, 1986

Microtubule associated tau protein induction by nerve growth factor during neurite outgrowth in pc 12 cells. Borisy, G G , D W Cleveland And D B Murphy (Ed ) Molecular Biology Of The Cytoskeleton; Meeting, Cold Spring Harbor, N Y , Usa, Apr Xvi+512p Cold Spring Harbor Laboratory: Cold Spring Harbor, N Y , Usa Illus 343-356, 1984

Depolarization of PC12 cells induces neurite outgrowth and enhances nerve growth factor-induced neurite outgrowth in rats. Neuroscience Letters 351(1): 41-45, 2003

Cytotoxic effects of acrylamide in nerve growth factor or fibroblast growth factor 1-induced neurite outgrowth in PC12 cells. Archives of Toxicology 88(3): 769-780, 2014

Regulation of microtubule composition and stability during nerve growth factor-promoted neurite outgrowth. Journal of Cell Biology 103(2): 545-557, 1986

Sertraline inhibits nerve growth factor-induced neurite outgrowth in PC12 cells via a mechanism involving the sigma-1 receptor. European Journal of Pharmacology 853: 129-135, 2019

Nerve growth factor induces neurite outgrowth of PC12 cells by promoting Gβγ-microtubule interaction. Bmc Neuroscience 15: 132, 2014

Neurotropin inhibits both capsaicin-induced substance P release and nerve growth factor-induced neurite outgrowth in cultured rat dorsal root ganglion neurones. Clinical and Experimental Dermatology 35(1): 73-77, 2010