EurekaMag
+ Translate
+ Most Popular
Cunninghamia lanceolata plantations in China
Mammalian lairs in paleo ecological studies and palynology
Studies on technological possibilities in utilization of anhydrous milk fat for production of recombined butter-like products
Should right-sided fibroelastomas be operated upon?
Large esophageal lipoma
Apoptosis in the mammalian thymus during normal histogenesis and under various in vitro and in vivo experimental conditions
Poissons characoides nouveaux ou non signales de l'Ilha do Bananal, Bresil
Desensitizing efficacy of Colgate Sensitive Maximum Strength and Fresh Mint Sensodyne dentifrices
Administration of fluid by subcutaneous infusion: revival of a forgotten method
Tundra mosquito control - an impossible dream?
Schizophrenia for primary care providers: how to contribute to the care of a vulnerable patient population
Geochemical pattern analysis; method of describing the Southeastern limestone regional aquifer system
Incidence of low birth weights in a hospital of Mexico City
Tabanidae
Graded management intensity of grassland systems for enhancing floristic diversity
Microbiology and biochemistry of cheese and fermented milk
The ember tetra: a new pygmy characid tetra from the Rio das Mortes, Brazil, Hyphessobrycon amandae sp. n. (Pisces, Characoidei)
Risk factors of contrast-induced nephropathy in patients after coronary artery intervention
Renovation of onsite domestic wastewater in a poorly drained soil
Observations of the propagation velocity and formation mechanism of burst fractures caused by gunshot
Systolic blood pressure in a population of infants in the first year of life: the Brompton study
Haematological studies in rats fed with metanil yellow
Studies on pasteurellosis. I. A new species of Pasteurella encountered in chronic fowl cholera
Dormancy breaking and germination of Acacia salicina Lindl. seeds
therapy of lupus nephritis. a two-year prospective study

Major histocompatibility complex class II-restricted antigen presentation across a species barrier: conservation of restriction determinants in evolution


Major histocompatibility complex class II-restricted antigen presentation across a species barrier: conservation of restriction determinants in evolution



Journal of Experimental Medicine 172(1): 53-59



ISSN/ISBN: 0022-1007

PMID: 1694228

DOI: 10.1084/jem.172.1.53

The existence of at least three alleles of the HLA-DRB3 gene within the human population is evident. These alleles express DRw52 determinants and react with monoclonal antibody (mAb) 7.3.19.1. The polymorphic epitope recognized by 7.3.19.1 is not only present on human cells but is also expressed on chimpanzee (Pan troglodytes) class II-positive cells. The 7.3.19.1 determinant already existed before speciation of man and chimpanzee, and is at least 5,000,000 yr old. Two-dimensional gel electrophoresis demonstrated that the various HLA- and Patr-DRw52 molecules that are reactive with 7.3.19.1 exhibit isoelectric point differences due to primary amino acid heterogeneity, as was confirmed by sequencing data. Sequence comparison allowed us to map the binding site of mAb 7.3.19.1 to the alpha helix of the major histocompatibility complex (MHC) class II DRB1 domain surrounding the antigen-binding cleft. Despite MHC sequence variation, chimpanzee antigen-presenting cells can present antigen (purified protein derivative) to human T cell lines and vice versa. Only the HLA- and Patr-DRw52 molecules were shown to function as restriction elements for antigen presentation across this species barrier. It is concluded that these particular restriction determinants probably have been conserved in evolution. The HLA- and Patr-DRw52 molecules represent alleles displaying polymorphism that has been selected for in evolution. Such "biomutants" may thus be more useful to study the biological significance of MHC molecules than MHC variants that have been generated by in vitro mutagenesis experiments.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 040625090

Download citation: RISBibTeXText

Related references

Major histocompatibility complex class II-restricted presentation of an internally synthesized antigen displays cell-type variability and segregates from the exogenous class II and endogenous class I presentation pathways. Journal of Experimental Medicine 178(1): 73-85, 1993

Major histocompatibility complex class I-restricted antigen processing and presentation. Tissue Antigens 60(1): 1-9, 2002

Delivery of protein antigen to the major histocompatibility complex class I-restricted antigen presentation pathway. Journal of Drug Targeting 3(2): 91-109, 1995

Class II major histocompatibility complex-restricted T cells specific for a virion structural protein that do not recognize exogenous influenza virus. Evidence that presentation of labile T cell determinants is favored by endogenous antigen synthesis. Journal of Experimental Medicine 169(3): 921-931, 1989

Class Ii major histocompatibility complex-restricted T cells specific for a virion structural protein that do not recognize exogenous influenza virus. Evidence that presentation of labile T cell determinants is favored by endogenous antigen synthesis. Journal of Experimental Medicine 169(3): 921-931, 1989

The role of H2-O and HLA-DO in major histocompatibility complex class II-restricted antigen processing and presentation. Immunological Reviews 172: 255-266, 1999

The role of H2-O in major histocompatibility complex class II-restricted antigen processing and presentation. FASEB Journal 14(6): A1159, 2000

Major histocompatibility complex class II-restricted presentation of a cytosolic antigen by autophagy. European Journal of Immunology 33(5): 1250-1259, 2003

The role of H2-O and HLA-DO in major histocompatibility complex class Il-restricted antigen processing and presentation. Immunological Reviews 172(1): 255-266, 1999

Functional analysis of antigen processing and major histocompatibility complex class II-restricted presentation. Methods in Molecular Biology 156: 49-56, 2001

A gene required for class II-restricted antigen presentation maps to the major histocompatibility complex. Journal of Experimental Medicine 174(6): 1607-1615, 1991

Thiol oxidation and reduction in major histocompatibility complex class I-restricted antigen processing and presentation. Methods in Enzymology 348: 49-54, 2002

Mechanisms of major histocompatibility complex class II-restricted processing and presentation of the V antigen of Yersinia pestis. Immunology 119(3): 385-392, 2006

Dichotomy in antigen presentation requirements of class i and class ii major histocompatibility complex restricted influenza hemagglutinin specific murine cytolytic t lymphocyte clones. Federation Proceedings 45(4): 979, 1986

Pretransplant detection of human minor histocompatibility antigen-specific naive and memory interleukin-2-secreting T cells within class I major histocompatibility complex (MHC)-restricted CD8+ and class II MHC-restricted CD4+ T-cell subsets. Blood 82(1): 298-306, 1993