Section 41
Chapter 40,631

Malignant transformation of human bladder epithelial cells by DNA transfection with the v-raf oncogene

Skouv, J.; Ottesen, S.; Mark, G.; Autrup, H.

Molecular Carcinogenesis 2(2): 59-62


ISSN/ISBN: 0899-1987
PMID: 2548527
DOI: 10.1002/mc.2940020202
Accession: 040630284

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Transfection of the v-raf oncogene into immortalized, nontumorigenic human bladder epithelial cells resulted in the isolation of two tumorigenic transformants. Both were identified as human and of the same origin as the parent cell line by human leukocyte antigen typing and Southern blot analysis. Both the primary tumorigenic transfectants and the cell lines established from the induced tumors expressed v-raf mRNA and v-raf protein. In both tumorigenic transformants the level of c-myc mRNA was enhanced compared with that of the parent cell line.

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