+ Site Statistics
+ Search Articles
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on Google+Follow on Google+
Follow on LinkedInFollow on LinkedIn

+ Translate

Measurement of the S-phase duration in normal and abnormal human endometrium by in vitro double labelling with bromodeoxyuridine and tritiated thymidine

Journal of Pathology 157(2): 109-115

Measurement of the S-phase duration in normal and abnormal human endometrium by in vitro double labelling with bromodeoxyuridine and tritiated thymidine

The duration of the S phase of the cell cycle (ts) in the glands and stroma of normal, hyperplastic, and malignant endometrium was measured in vitro using a double labelling technique. Endometrial organ cultures were incubated sequentially in tritiated thymidine (3HTdR) and bromodeoxyuridine (BrdU) each for 1 h. Labelled cells were visualized in tissue sections using 3HTdR autoradiography and immunohistochemistry with an anti-(BrdU) antibody. Three populations of cells, single labelled with either 3HTdR or BrdU, and double labelled cells, were easily identified and ts was calculated from both the rate of influx into, and efflux out of S. There were no significant differences in ts in the different tissue types or between influx and efflux in the glands. However, in the stroma ts calculated from the rate of efflux was significantly shorter than that calculated from the rate of influx, suggesting that during the cell culture period the rate of cell efflux from S was greater than influx. This is the opposite to what would be expected in an exponentially growing cell population and suggests that there may be an effect from the culturing conditions.

(PDF same-day service: $19.90)

Accession: 040653365

PMID: 2921671

DOI: 10.1002/path.1711570205

Related references

Snap labelling of s phase cells with bromodeoxyuridine in normal hyperplastic and malignant human endometrium a comparison with tritiated thymidine labelling. Journal of Pathology 152(3): 191A, 1987

Flash labelling of S-phase cells in short-term organ culture of normal and pathological human endometrium using bromodeoxyuridine and tritiated thymidine. Journal of Pathology 154(4): 321-328, 1988

Estimation of S phase duration in goat epidermis by an in vivo intradermal double labelling technique using bromodeoxyuridine and tritiated thymidine. Research in veterinary science 52(1): 5-9, 1992

Double labelling of cells with tritiated thymidine and bromodeoxyuridine reveals a circadian rhythm-dependent variation in duration of DNA synthesis and S phase flux rates in rodent oral epithelium. Cell and Tissue Kinetics 23(4): 313-323, 1990

Double labelling of tissue combining tritiated thymidine autoradiography with immunodetection of bromodeoxyuridine the autoradiographic significance of inhibition of thymidine incorporation into dna by bromodeoxyuridine given simultaneously. Cell & Tissue Kinetics 22(5): 393-399, 1989

Measurement of S phase duration in human epidermis using cyclin immunostaining and tritiated-thymidine pulse labelling. Archives of Dermatological Research 284(4): 238-241, 1992

S phase duration measurement by combined pcna cyclin immunostaining and radioautography after a single pulse labelling with tritiated thymidine. Cell Biology International Reports 14(9): 765-774, 1990

Tritiated thymidine and bromodeoxyuridine double-labelling studies on growth factors and oral epithelial proliferation in the mouse. Archives of Oral Biology 44(9): 721-734, Sept, 1999

Double labelling with bromodeoxyuridine and tritiated thymidine of proliferative cells in small intestinal epithelium in steady state and after irradiation. Cell & Tissue Kinetics 21(5): 317-330, 1988

DNA-synthesizing cells in oral epithelium have a range of cell cycle durations: evidence from double-labelling studies using tritiated thymidine and bromodeoxyuridine. Cell and Tissue Kinetics 22(5): 377-382, 1989