+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Mechanism of airway narrowing caused by inhaled platelet-activating factor. Role of airway microvascular leakage



Mechanism of airway narrowing caused by inhaled platelet-activating factor. Role of airway microvascular leakage



American Review of Respiratory Disease 143(6): 1345-1349



In order to study the mechanism of airway narrowing after inhaled platelet-activating factor (PAF) we measured concomitant changes in lung resistance (RL) and in airway microvascular leakage in anesthetized guinea pigs. RL and its recovery after hyperinflation at 5 min were measured until 6 min after PAF aerosol (0.1, 0.3, 1, and 3 mM), and in the case of 3 mM PAF also until 10 min. Microvascular leakage in trachea, main bronchi, and proximal and distal intrapulmonary airways was determined by measurement of extravasated Evans blue dye content. For comparison, the responses to inhaled histamine (3 mM) and 5-hydroxytryptamine (5HT) (3 mM), which act directly on airway smooth muscle, were also examined. Inhaled PAF increased RL dose-dependently, with a maximal response (peak RL) at 4 min after the inhalation, whereas the response to histamine or 5HT was maximal within a few seconds after the inhalation. Peak RL (cm H2O/ml/s) was significantly less after PAF (1.03 +/- 0.09) than after histamine (8.39 +/- 1.07) or 5HT (18.3 +/- 6.48), although there was no significant difference in RL after hyperinflation (recovery RL). No additional increase in RL was seen between 5 and 10 min after exposure. PAF caused a dose-dependent increase in Evans blue dye extravasation; 3 mM PAF induced significantly higher leakage than did histamine or 5HT at all airway levels at 6 min. PAF did not cause any additional extravasation of Evans blue dye at 10 min compared with that at 6 min after exposure.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 040657795

Download citation: RISBibTeXText

PMID: 2048823

DOI: 10.1164/ajrccm/143.6.1345


Related references

Role of thromboxane A2 in airway microvascular leakage induced by inhaled platelet-activating factor. Journal of Applied Physiology 71(5): 1729-1734, 1991

Inhaled rolipram and zaprinast inhibit airway microvascular leakage produced by inhaled platelet-activating factor in the guinea-pig. British Journal of Pharmacology 112(PROC SUPPL ): 592P, 1994

Effect of inhaled lyso-platelet-activating factor on airway microvascular leakage in the guinea pig. Journal of Applied Physiology 74(3): 1117-1122, 1993

Bradykinin induced airway narrowing and airway narrowing and airway microvascular leakage in guinea pig role of cholinergic nerves and of arachidonic acid metabolites. American Review of Respiratory Disease 145(4 PART 2): A612, 1992

Inhaled platelet-activating factor increases airway sensitivity but not maximal airway narrowing to methacholine in normal subjects. Monaldi Archives for Chest Disease 49(4): 288-292, 1994

Bradykinin causes airway hyperresponsiveness and enhances maximal airway narrowing. Role of microvascular leakage and airway edema. American Review of Respiratory Disease 146(5 Pt 1): 1301-1305, 1992

Effect of A Platelet Activating Factor (PAF) Antagonist (Web 2086) on Paf Induced Airway Microvascular Leakage and Platelet Aggregation. Clinical Science 72(S16): 68p.1-68p, 1987

Involvement of platelet activating factor in airway microvascular leakage induced by endotoxin. American Review of Respiratory Disease 137(4 PART 2): 236, 1988

Platelet activating factor induced airway microvascular leakage effects of paf antagonists and antiasthma drugs. Handley, D A , Et Al (Ed ) Inflammatory Disease And Therapy, Vol 3 Platelet-Activating Factor in Endotoxin And Immune Diseases Xvii+692p Marcel Dekker, Inc : New York, New York, Usa; Basel, Switzerland Illus 307-328, 1990

Effects of platelet activating factor paf antagonist web 2086 on airway microvascular leakage induced by paf and antigen. American Review of Respiratory Disease 135(4 PART 2): A162, 1987

Platelet-activating factor mediates the ozone-induced increase in airway microvascular leakage in guinea pigs. European Journal of Pharmacology Environmental Toxicology & Pharmacology Section 4(3-4): 251-255, 1995

Effect ofβ(in2)-adrenoceptor agonists against platelet-activating factor-induced airway microvascular leakage and bronchoconstriction in the guinea pig. Inflammation Research 40(1-2): 50-56, 1993

Effect of beta 2-adrenoceptor agonists against platelet-activating factor-induced airway microvascular leakage and bronchoconstriction in the guinea pig. Agents and Actions 40(1-2): 50-56, 1993

Rolipram inhibits airway microvascular leakage induced by platelet-activating factor, histamine and bradykinin in guinea-pigs. Journal of Pharmacy and Pharmacology 45(12): 1090-1092, 1993

Comparative effects of tetrandrine and berbamine on guineapig airway microvascular leakage induced by platelet-activating factor and other allergic mediators. International Journal of Immunopharmacology 15(2): 185-193, 1993