+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Metabolic studies on the uptake of [14C]-histidine and [14C]-histamine and histamine synthesis by guinea-pig basophils, in vitro



Metabolic studies on the uptake of [14C]-histidine and [14C]-histamine and histamine synthesis by guinea-pig basophils, in vitro



Immunology 36(3): 539-548



The uptake of [14C]-histidine and [14C]-histamine and the conversion of [14C]-histidine to [14C]-histamine was measured in suspensions of guinea-pig bone marrow cells rich in basophils. When comparable amounts of labelled histidine or histamine were added to equal numbers of basophils, the uptake of histidine was approximately forty-five times greater than that of histamine. Purified eosinophils, neutrophils and mononuclear cells incorporated only a small proportion of [14C]-histidine when compared to the basophil; [14C]-histamine uptake by all these cell types was virtually negligible. Histidine uptake and the amount of histamine formed de novo was directly related to the number of basophils, the time of incubation and the substrate concentration. Histidine uptake was decreased by agents which inhibit glycolysis, oxidative phosphorylation, Na + - K + -dependent ATPase, protein synthesis and RNA synthesis. Inhibition was demonstrable in a dose-dependent fashion and at concentrations which had no apparent effect on cell viability. Inhibitors of DNA synthesis, and of microtubule function, had no influence on histidine uptake. Cytochalasin B, an inhibitor of microfilament function, also decreased histidine uptake but only at concentrations previously showen to affect hexose transport. None of the agents tested affected the uptake of [14C]-histamine or the amounts of new histamine formed from the histidine that had been incorporated. These studies suggest that histidine is preferentially incorporated into the basophil; that the uptake depends on the integrity of a number of metabolic pathways, but that once the histidine is taken up these requirements do not apply to the formation of new histamine. In contrast, histamine appeared to diffuse passively, and in relatively small amounts, into all the cell types tested.

Please choose payment method:






(PDF emailed within 1 workday: $29.90)

Accession: 040683089

Download citation: RISBibTeXText

PMID: 437843


Related references

Regulation of histamine synthesis metiamide induced synthesis of histamine in the rat stomach and the effect of histidine decarboxylase inhibitors on the elevated carbon 14 histamine level and histidine decarboxylase activity. Agents & Actions 8(4): 393-394, 1978

Histamine increases histidine uptake by basophils. Clinical and Experimental Immunology 40(2): 423-426, 1980

Regulation of histamine synthesis: Metiamide-induced synthesis of histamine in the rat stomach and the effect of histidine decarboxylase inhibitors on the elevated14C-histamine level and histidine decarboxylase activity. Inflammation Research 8(4): 393-394, 1978

Regulation of histamine synthesis: metiamide-induced synthesis of histamine in the rat stomach and the effect of histidine decarboxylase inhibitors on the elevated 14C-histamine level and histidine decarboxylase activity. Agents and Actions 8(4): 393-394, 1978

A mononuclear cell derived histamine releasing factor (HRF) in asthmatic patients. Histamine release from basophils in vitro. Annals of Allergy 53(1): 66-69, 1984

Histidine decarboxylase activity and histamine level in wounds of guinea pigs treated with histamine liberator 48 80. Acta Physiologica Polonica (English Translation) TT(69-50009-3): 432-433, 1969

Interaction of histamine H1-and H2-receptor antagonists with histamine uptake and metabolism by guinea-pig isolated atrium and mouse neoplastic mast cells cells in vitro. British Journal of Pharmacology 53(4): 569-574, 1975

Histamine synthesis by guinea pig basophils in short term tissue culture. Federation Proceedings 34(3): 1045, 1975

Long-term depletion of histamine in guinea-pigs by administration of α-fluoromethylhistidine, a specific inhibitor of histidine decarboxylase; effect on the sensitivity of histamine receptors. Inflammation Research 24(1-2): 56-64, 1988

Regulation of histamine synthesis altered synthesis and level of histamine in the hypothalamus rats by repeated administration of histamine h 1 and histamine h 2 receptor antagonists. Agents & Actions 10(1-2): 98-100, 1980

Long-term depletion of histamine in guinea-pigs by administration of alpha-fluoromethylhistidine, a specific inhibitor of histidine decarboxylase; effect on the sensitivity of histamine receptors. Agents and Actions 24(1-2): 56-64, 1988

Further studies on the process of histamine release from sensitized guinea-pig bone marrow basophils by antigen. International Archives of Allergy and Applied Immunology 37(4): 376-384, 1970

Studies on histamine with L-histidine decarboxylase, a histamine-forming enzyme, as a probe: from purification to gene knockout. Nihon Yakurigaku Zasshi. Folia Pharmacologica Japonica 118(3): 159-169, 2001

In vitro inhibition, by loratadine and descarboxyethoxyloratadine, of histamine release from human basophils, and of histamine release and intracellular calcium fluxes in rat basophilic leukemia cells (RBL-2H3). Biochemical Pharmacology 47(5): 789-794, 1994

Metabolic studies on histidine, histamine, and related imidazoles. Pharmacological Reviews 6(3): 299-343, 1954