Mitotic spindle damage induced by 3,4-dichloroaniline in V79 Chinese hamster cells examined by differential staining of the spindle apparatus and chromosomes

Salassidis, K.; Bauchinger, M.

Mutagenesis 5(4): 367-370

1990


ISSN/ISBN: 0267-8357
PMID: 1697924
DOI: 10.1093/mutage/5.4.367
Accession: 040709307

Download citation:  
Text
  |  
BibTeX
  |  
RIS

Article/Abstract emailed within 0-6 h
Payments are secure & encrypted
Powered by Stripe
Powered by PayPal

Abstract
3,4-Dichloroaniline (3,4-DCA), an intermediate in various chemical syntheses, has been detected as an environmental contaminant in surface waters and in the effluents from dye manufacturing plants. Previously we showed that the compound caused mitotic spindle damage. In the present study a preparation and staining technique has been used that allows a direct examination of the spindle apparatus and chromosome behaviour of 3,4-DCA-exposed V79 Chinese hamster cells. After a 3 h exposure the compound caused monopolar mitoses and metaphase arrest in a dose-dependent manner, most probably by interfering with centriole separation. At a concentration of 1 mM, increased levels of aneuploid cells could be observed in cells exposed for two cell division cycles.