+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Modulation of polyunsaturated fatty acid content of triglycerides in rat pre-adipocytes in culture

Modulation of polyunsaturated fatty acid content of triglycerides in rat pre-adipocytes in culture

Lipids 26(9): 705-710

Rat peri-renal and epididymal pre-adipocytes in culture undergoing triglyceride (TG) accumulation were incubated with oleic (18:1), linoleic (18:2), alpha-linolenic (18:3 omega 3), arachidonic (20:4) and 4,7,10,13,16,19-docosahexaenoic (22:6 omega 3) acids in the presence of 0.8 microM insulin. The fatty acids were incorporated in cellular TG with relative enrichments over control from 1.4-fold for 18:1 to greater than 40-fold for 18:3 omega 3. Greater than 80% of fatty acids taken up were incorporated into cellular TG. The balance was distributed, in decreasing amounts, into phospholipids, unidentified intracellular constituents, and ketone bodies. The P/S ratio of cellular TG was at least an order of magnitude lower than that of the external milieu for both cell types and for all treatment groups, including controls. Doubling the concentration of treatment fatty acid increased its incorporation into cellular TG. However, it did not affect the accumulation of the other fatty acids in TG. Epididymal cells consistently acquire a higher proportion of treatment fatty acids in cell TG than peri-renal cells. Pre-adipocytes with polyunsaturated fatty acids (PUFA)-enriched TG is a potential model for the study of PUFA metabolism in these types of cells.

Please choose payment method:

(PDF emailed within 0-6 h: $19.90)

Accession: 040719732

Download citation: RISBibTeXText

PMID: 1762515

DOI: 10.1007/bf02535618

Related references

Levels of polyunsaturated fatty acids in lymphocytes of rats fed on diets varying in polyunsaturated fatty acid content. British Journal of Nutrition 43(2): 367-373, 1980

Transcriptional regulation of fatty acid synthase gene by insulin/glucose, polyunsaturated fatty acid and leptin in hepatocytes and adipocytes in normal and genetically obese rats. European Journal of Biochemistry 260(2): 505-511, 1999

Modulation of responses of lung and liver to tnf alpha by fats of various saturated monounsaturated and polyunsaturated fatty acid content. Cytokine 3(5): 509, 1991

Method of producing a polyunsaturated fatty acid containing culture and polyunsaturated fatty acid containing oil using microorganisms. Official Gazette of the United States Patent & Trademark Office Patents 1263(2), 2002

Changes in the lipid composition and maximisation of the polyunsaturated fatty acid content of three microalgae grown in mass culture. Journal of Applied Phycology 5(1): 71-83, 1993

Dietary (n-3) and (n-6) polyunsaturated fatty acids rapidly modify fatty acid composition and insulin effects in rat adipocytes. Journal of Nutrition 128(3): 512-519, 1998

Perturbation and age-related changes in the fatty acid pattern of soleus muscle phospholipids and triglycerides in rats depleted in long-chain polyunsaturated omega3 fatty acids. International Journal of Molecular Medicine 20(6): 897-904, 2007

The production of fish oils enriched in polyunsaturated fatty acid-containing triglycerides. Journal of the American Oil Chemists' Society 70(2): 133-138, 1993

Modulation of human postprandial lipemia by changing ratios of polyunsaturated to saturated (P/S) fatty acid content of blended dietary fats: a cross-over design with repeated measures. Nutrition Journal 12: 122, 2013

Synthesis of n 3 polyunsaturated fatty acid rich triglycerides by immobilized microbial lipase. Journal of the Japan Oil Chemists' Society 41(1): 39-42, 1992

Synthesis of polyunsaturated fatty acid-enriched triglycerides by lipase-catalyzed esterification. Journal of the American Oil Chemists' Society 75(10): 1329-1337, 1998

Fatty aldehyde dehydrogenase is up-regulated by polyunsaturated fatty acid via peroxisome proliferator-activated receptor alpha and suppresses polyunsaturated fatty acid-induced endoplasmic reticulum stress. Febs Journal 276(23): 6956-6970, 2009

Omega-3 polyunsaturated fatty acid has an anti-oxidant effect via the Nrf-2/HO-1 pathway in 3T3-L1 adipocytes. Biochemical and Biophysical Research Communications 430(1): 225-230, 2013

The preparation of triglycerides highly enriched with omega 3 polyunsaturated fatty acid via lipase catalyzed interesterification. Tetrahedron Letters 30(13): 1671-1674, 1989

Diet (n-3) polyunsaturated fatty acid content and parity interact to alter maternal rat brain phospholipid fatty acid composition. Journal of Nutrition 136(8): 2236-2242, 2006