Monitoring and risk assessment by means of alkyl groups in hemoglobin in persons occupationally exposed to ethylene oxide
Calleman, C.J.; Ehrenberg, L.; Jansson, B.; Osterman-Golkar, S.; Segerbäck, D.; Svensson, K.; Wachtmeister, C.A.
Journal of Environmental Pathology and Toxicology 2(2): 427-442
ISSN/ISBN: 0146-4779 PMID: 739221 Accession: 040727774
In persons occupationally exposed to ethylene oxide, i.e. under the conditions described by Dunkelberg and Hartmetz (1977), the degree of alkylation in histidine of hemoglobin was determined. Quantitative determination of N-3-(2-hydroxyethyl)histidine by mass fragmentography and by ion-exchange amino-acid analysis gave consistent results. Data are in agreement with the fast elimination from tissues (lambda = 4.6 hr-1, i.e. biological half-life about 9 min) found in the mouse. At the respiration rate of light work, and exposure dose of 1 ppm/hr results in a tissue dose that is estimated to involve a risk amounting to 1.101 mrad-equivalents of stochastic effects with a genetic mechanism.