+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Monoclonal antipeptide antibodies recognize IL-3 and neutralize its bioactivity in vivo



Monoclonal antipeptide antibodies recognize IL-3 and neutralize its bioactivity in vivo



Journal of Immunology 140(4): 1182-1187



Mixtures of synthetic peptides corresponding to segments of murine IL-3 or synthetic IL-3 were used to raise murine mAb. Several mAb able to recognize synthetic IL-3 were obtained, two of which exhibited significant cross-reactivity with native IL-3 as shown by precipitation of biosynthetically 35S-labeled IL-3 and their effectiveness as affinity reagents for the purification of IL-3 from conditioned medium. The amino acid sequence recognized by the two mAb was determined by using synthetic peptide segments of IL-3. In both cases binding of the mAb to synthetic IL-3 was inhibited best with a hexapeptide corresponding to the amino acid residues 130-135 of IL-3, although the mAb differed in other characteristics. Neither mAb neutralized IL-3 bioactivity in vitro. However, we observed that in vivo administration of one mAb abrogated the increase in splenic mast cells and their precursors that normally occurred in mice bearing a s.c. IL-3-producing tumor, WEHI-3B.

Please choose payment method:






(PDF emailed within 1 workday: $29.90)

Accession: 040731377

Download citation: RISBibTeXText

PMID: 3257773


Related references

Antipeptide antibodies of predetermined specificity recognize and neutralize the bioactivity of the pan-specific hemopoietin interleukin 3. Journal of Immunology 138(4): 1099-1104, 1987

Monoclonal antibodies that recognize human granulocyte-macrophage colony-stimulating factor and neutralize its bioactivity in vitro. Hybridoma 9(6): 545-558, 1990

Monoclonal antibodies specific for rat relaxin. I. Production and characterization of monoclonal antibodies that neutralize rat relaxin's bioactivity in vivo. Endocrinology 123(5): 2472-2478, 1988

Anti-idiotypic antibodies to monoclonal antibodies that neutralize coxsackievirus B4 do not recognize viral receptors. Virology 150(2): 352-360, 1986

Development of rat anti-mouse interleukin 3 monoclonal antibodies which neutralize bioactivity in vitro. Journal of Immunology 140(1): 131-137, 1988

Monoclonal antipeptide antibodies recognize epitopes upon VP4 and VP7 of simian rotavirus SA11 in infected MA104 cells. Archives of Virology 122(3-4): 281-291, 1992

Monoclonal antibodies that neutralize HEV recognize an antigenic site at the carboxyterminus of an ORF2 protein vaccine. Vaccine 22(2): 257-267, 2003

Characterization of murine monoclonal antibodies that recognize defined epitopes of pertussis toxin and neutralize its toxic effect on Chinese hamster ovary cells. Infection and Immunity 59(11): 4249-4251, 1991

Anti-erythropoietin receptor (EPO-R) monoclonal antibodies inhibit erythropoietin binding and neutralize bioactivity. Blood 82(1): 46-52, 1993

Two antipeptide monoclonal antibodies that recognize adhesive sequences in fibrinogen: identification of antigenic determinants and unrelated sequences using synthetic combinatorial libraries. Biomedical Peptides Proteins and Nucleic Acids 1(3): 199-204, 1995

Monoclonal antibodies recognize conformational alterations leading to decreased bioactivity of human growth hormone. Molecular & Cellular Endocrinology 38(1): 75-80, 1984

Isolation and characterization of monoclonal antibodies which neutralize human metapneumovirus in vitro and in vivo. Journal of Virology 80(16): 7799-7806, 2006

Antipeptide antibodies recognize both a substance p sp receptor and anti sp antibodies. FASEB Journal 3(3): A732, 1989

Antipeptide antibodies directed to the C-terminal part of ammodytoxin a react with the PLA-2 subunit of crotoxin and neutralize its pharmacological activity. Toxicon. 32(11): 1337-1348, 1994