+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Mucosal and systemic antibody responses to bovine coronavirus structural proteins in experimentally challenge-exposed calves fed low or high amounts of colostral antibodies



Mucosal and systemic antibody responses to bovine coronavirus structural proteins in experimentally challenge-exposed calves fed low or high amounts of colostral antibodies



American Journal of Veterinary Research 52(5): 700-708



Ten colostrum-deprived calves were assigned to 1 of 2 treatment groups (5 calves/group), and fed colostrum that had either low (naturally infected cows) or high (immunized cows) antibody titers to bovine coronavirus (BCV). All calves were inoculated orally and intranasally with virulent BCV when they were 24 to 48 hours old and challenge exposed 21 days later. Blood, feces, nasal secretions, tears, saliva, and bronchoalveolar lavage (BAL) fluids were collected weekly from each calf for 5 weeks after inoculation. The titers to whole BCV or the relative amounts of isotype-specific antibodies to BCV structural proteins were evaluated in these samples by ELISA or immunoblotting, respectively. Both pools of colostrum contained primarily IgG1, IgG2, and IgA antibodies to the E2 and E3 BCV proteins. Calves fed the high-titer colostrum had correspondingly higher amounts of passive IgG1 and IgA antibodies to whole BCV and to the E2 and E3 BCV proteins in serum, feces, and BAL fluid at postinoculation week 1 than those calves fed low-titer colostrum. Active IgG1, IgA, and IgM antibody responses in serum and active IgA and IgM antibody responses in most mucosal secretions to whole BCV and to the E2 and E3 proteins were lower or delayed in calves fed high-titer colostrum, compared with responses in calves fed low-titer colostrum. In contrast, increased responses to the BCV N protein were observed in all samples (except in serum and BAL fluid) in the calves fed high-titer colostrum, compared with calves fed low-titer colostrum. Upon challenge exposure, responses to E2 and E3 BCV proteins in serum and BAL fluid were lower in the group fed high-titer colostrum, compared with those in the group fed low-titer colostrum. Our findings indicate that the level of passive immunity in calves at the time of BCV inoculation can influence the development of active antibody responses in serum, feces, and mucosal secretions to whole BCV and to some BCV proteins individually.

Please choose payment method:






(PDF emailed within 1 workday: $29.90)

Accession: 040746932

Download citation: RISBibTeXText

PMID: 1649563


Related references

Isotype-specific antibody responses to bovine coronavirus structural proteins in serum, feces, and mucosal secretions from experimentally challenge-exposed colostrum-deprived calves. American Journal of Veterinary Research 52(5): 692-699, 1991

Mucosal and systemic isotype-specific antibody responses to bovine coronavirus structural proteins in naturally infected dairy calves. American Journal of Veterinary Research 52(6): 852-857, 1991

Modulation by colostrum-acquired maternal antibodies of systemic and mucosal antibody responses to rotavirus in calves experimentally challenged with bovine rotavirus. VeterinaryImmunologyandImmunopathology100(1-2):7, 2004

Milk supplemented with immune colostrum: protection against rotavirus diarrhea and modulatory effect on the systemic and mucosal antibody responses in calves experimentally challenged with bovine rotavirus. Veterinary Immunology and Immunopathology 136(1-2): 12-27, 2010

Isotype-specific antibody responses in sera and mucosal secretions of calves experimentally infected with bovine herpesvirus 1. Veterinary Immunology & Immunopathology 46(3-4): 267-283, 1995

Comparison of bovine serum and colostral antibody: effect of colostral antibody on vaccination of calves for contagious bovine pleuropneumonia. Immunology 18(3): 369-377, 1970

Bovine coronavirus in naturally and experimentally exposed calves; viral shedding and the potential for transmission. Virology Journal 13: 100, 2016

Epidemiologic factors and isotype-specific antibody responses in serum and mucosal secretions of dairy calves with bovine coronavirus respiratory tract and enteric tract infections. American Journal of Veterinary Research 52(6): 845-851, 1991

Evaluation of the effect of serum antibody abundance against bovine coronavirus on bovine coronavirus shedding and risk of respiratory tract disease in beef calves from birth through the first five weeks in a feedlot. American Journal of Veterinary Research 78(9): 1065-1076, 2017

Antibody responses in spiral colon, ileum, and jejunum of bovine coronavirus-infected neonatal calves. Comparative Immunology Microbiology & Infectious Diseases 17(2): 139-149, 1994

Immune response of pregnant heifers and cows to bovine rotavirus inoculation and passive protection to rotavirus infection in newborn calves fed colostral antibodies or colostral lymphocytes. American Journal of Veterinary Research 49(7): 1084-1091, 1988

Effects of dietary vitamin A content on antibody responses of feedlot calves inoculated intramuscularly with an inactivated bovine coronavirus vaccine. American Journal of Veterinary Research 74(10): 1353-1362, 2013

Experimental infection of calves with Newcastle disease virus induces systemic and mucosal antibody responses. Archives of Virology 153(6): 1197-1200, 2008

Bovine parainfluenza-3 virus proteins recognized by antibodies of aerosol-exposed calves. American Journal of Veterinary Research 49(11): 1945-1949, 1988

Additive protective effects of colostral antipili antibodies in calves experimentally infected with enterotoxigenic Escherichia coli. Infection and Immunity 50(3): 947-949, 1985