+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

N-independent leftward transcription in coliphage lambda: deletions, insertions and new promoters bypassing termination functions



N-independent leftward transcription in coliphage lambda: deletions, insertions and new promoters bypassing termination functions



Molecular and General Genetics 168(2): 211-230



Lambda mutants capable of N-independent red-gam gene expression were isolated by selecting Fec+ plaque-forming derivatives of lambda N+ nutL- (Fec-) strains. In addition to true nutL+ reversions, three classes of second-site mutations were identified: (1) ninL deletions that remove a region containing either tL1 or both tL1 and tL2 termination signals, or only a small region (defining the rut site) just upstream from tL1, (2) new constitutive promoters that map just upstream from the tL2 termination site and which are created either by point mutations (hip) or by short insertion sequences (isp), (3) small internal deletions in gene cro. The positions and individual effects of these mutations, some of which only partially abolish termination function, provide evidence for a complex multipartite structure of the termination signals.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 040767758

Download citation: RISBibTeXText

PMID: 286866

DOI: 10.1007/bf00431446


Related references

Transcription termination: sequence and function of the rho-independent tL3 terminator in the major leftward operon of bacteriophage lambda. Gene 17(3): 247-258, 1982

Regulation of leftward transcription in the J-b2-att region of coliphage lambda. Molecular and General Genetics 111(1): 22-34, 1971

Multiple pathways of RNA processing and decay for the major leftward N- independent RNA transcript of coliphage lambda. Virology 113(1): 39-53, 1981

Deletions and insertions in the immunity region of coliphage lambda: revised measurement of the promoter-startpoint distance. Virology 62(2): 458-471, 1974

Transcription termination sites in the major leftward operon of coli phage lambda. Virology 88(2): 252-262, 1978

The tL2 cluster of transcription termination sites between genes bet and ral of coliphage lambda. Virology 125(2): 403-418, 1983

Antitermination and termination functions of the cloned nutL, N, and tL1 modules of coliphage lambda. Gene 16(1-3): 261-274, 1981

Synthesis of the nutL DNA segments and analysis of antitermination and termination functions in coliphage lambda. Gene 18(3): 343-354, 1982

Initiation of coliphage lambda replication, lit, oop RNA synthesis, and effect of gene dosage on transcription from promoters PL, PR, and PR. Virology 97(2): 415-438, 1979

Processing of the major leftward mRNA of coliphage lambda. Virology 71(1): 262-277, 1976

The structure of the coliphage HK022 Nun protein-lambda-phage boxB RNA complex. Implications for the mechanism of transcription termination. Journal of Biological Chemistry 276(34): 32064-32070, 2001

Coli phage lambda nutl minus a unique class of mutants defective in the site of gene n product utilization for anti termination of leftward transcription. Journal of Molecular Biology 124(1): 195-222, 1978

A cluster of leftward, rho-dependent t'J terminators in the J gene of coliphage lambda. Gene 21(3): 175-191, 1983

Control of rightward transcription in coliphage lambda by the regulatory functions of phage genes N and cro. Virology 98(2): 424-432, 1979