+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Natural killer function in flow cytometry. III. Surface marker determination of K562-conjugated lymphocytes by dual laser flow cytometry



Natural killer function in flow cytometry. III. Surface marker determination of K562-conjugated lymphocytes by dual laser flow cytometry



Journal of Immunological Methods 149(2): 189-196



The recognition of effector cell populations that are able to actively from conjugates with target cells is of major importance in studies of lymphocyte cytotoxicity. A number of methodologies have been described to identify the conjugates and count them, but there have been few studies of the binding capability of the different subsets of effector cells involved in the conjugation phenomenon. Here we describe a methodology that permits the study of two surface markers on lymphocytes conjugated to K562 target cells. In particular, the expression of low density CD8 (CD8dim) has been studied on both CD3+ and CD16+ lymphocytes bound to K562 target cells. Previously described methodologies, either optical microscopy or flow cytometry, were not able to identify the effector population by mAb double staining, especially in the case of antigens expressed at low density. The flow cytometric methodology described here permits the measurement of the binding activity of small lymphocyte subsets such as the CD3+ 8dim+ population. However, the method could be used to study the binding activity of any effector population defined by mAb double staining.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 040775766

Download citation: RISBibTeXText

PMID: 1534339

DOI: 10.1016/0022-1759(92)90250-w


Related references

Natural killer function in flow cytometry. I. Evaluation of NK lytic activity on K562 cell line. Journal of Immunological Methods 107(1): 73-78, 1988

Natural killer function in flow cytometry: identification of human lymphoid subsets able to bind to the NK sensitive target K562. Cytometry 12(8): 717-722, 1991

Determination of natural killer cell function by flow cytometry. Clinical and Diagnostic Laboratory Immunology 3(3): 295-300, 1996

Kinetics of in vitro natural killer activity against K562 cells as detected by flow cytometry. Cytometry. 32(4): 280-285,. 1, 1998

Guidelines for performing surface antigen analysis on peripheral lymphocytes using flow cytometry by Japanese Committee for Clinical Laboratory Standards (JCCLS), Area Committee on Hematology, and Subcommittee on Flow Cytometry. Rinsho Byori. Japanese Journal of Clinical Pathology 47(11): 1027-1038, 1999

Natural killer activity in the rat. II. Analysis of surface antigens on LGL by flow cytometry. Journal of Immunology 127(6): 2204-2208, 1981

Phenotypic and functional study of natural killer lymphocytes in flow cytometry: application to renal transplantation. Annales de Biologie Clinique 48(9): 631-636, 1990

Development of a novel flow cytometry-based assay of natural killer cell function. Abstracts of the General Meeting of the American Society for Microbiology 99: 662, 1999

Determination of guanine-plus-cytosine content of bacterial DNA by dual-laser flow cytometry. Journal of General Microbiology 136(2): 359-365, 1990

Fixation and long-term storage of human lymphocytes for surface marker analysis by flow cytometry. Cytometry 9(3): 213-219, 1988

Laser flow cytometry and cancer chemotherapy: detection of intracellular anthracyclines by flow cytometry. Journal of Histochemistry and Cytochemistry 27(12): 1655-1656, 1979

Flow cytometry studies of a surface allo antigen specific for murine natural killer cells. Federation Proceedings: Abstract 2814, 1980

Quantitative determination of Bcl-2 in FACS-sorted normal and AML progenitor cells by laser scanning cytometry Comparison with flow cytometry and Western blot analysis. Annals of Hematology 80(Suppl. 2): S32, 2001

High affinity binding of fluorescein isothiocyanate to eosinophils detected by laser scanning cytometry: A potential source of error in analysis of blood samples utilizing fluorescein-conjugated reagents in flow cytometry. Cytometry 36(1): 77-82, 1999

Analysis of heavy metal immunotoxicity by multiparameter flow cytometry: correlation of flow cytometry and immune function data in B6CF1 mice. International Journal of Immunopharmacology 9(5): 597-610, 1987