+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Negative-strand RNA replication by Q beta and MS2 positive-strand RNA phage replicases



Negative-strand RNA replication by Q beta and MS2 positive-strand RNA phage replicases



Virology 178(1): 340-343



Plasmids were constructed which allowed preparation of positive- and negative-strand MS2 RNA by in vitro transcription and which expressed the MS2 phage replicase. Transcribed, positive-strand MS2 RNA was infectious to Escherichia coli spheroplasts. Transcribed, negative-strand MS2 RNA was infectious to spheroplasts containing either MS2 replicase or related bacteriophage Q beta replicase.

Please choose payment method:






(PDF emailed within 1 workday: $29.90)

Accession: 040779418

Download citation: RISBibTeXText

PMID: 2202149


Related references

Negative-strand RNA replication by Qb and MS2 positive-strand RNA phage replicases. Virology 178: 0-3, 1990

Single-stranded phage replication: positive- and negative-strand DNA synthesis. Cold Spring Harbor Symposia on Quantitative Biology 43 Pt 1: 349-365, 1979

Single stranded phage dna replication positive and negative strand dna synthesis. Cold Spring Harbor Symposia on Quantitative Biology : P48, 1979

RNA - protein interactions at the 3 end of poliovirus negative strand RNA involved in initiation of positive strand RNA replication. Journal of Cellular Biochemistry Suppl. 0(17 Part D): 13, 1993

Poly(A) at the 3' end of positive-strand RNA and VPg-linked poly(U) at the 5' end of negative-strand RNA are reciprocal templates during replication of poliovirus RNA. Journal of Virology 84(6): 2843-2858, 2010

Double-strand cleavage and strand joining by the replication initiator protein of filamentous phage f1. Journal of Biological Chemistry 264(21): 12627-12632, 1989

Negative strand RNA viruses come of age. Negative Strand RNA Virus Transcription and Replication sponsored by the Fundación Juan March, Madrid, Spain, April 22-24, 1991. New Biologist 3(8): 754-757, 1991

Importance of the positive-strand RNA secondary structure of a murine coronavirus defective interfering RNA internal replication signal in positive-strand RNA synthesis. Journal of Virology 72(10): 7926-7933, 1998

Two cis-acting elements in negative RNA strand of Hepatitis C virus involved in synthesis of positive RNA strand in vitro. Acta Virologica 49(2): 83-90, 2005

Positive-strand RNA produced from negative-strand RNA containing coat protein subgenomic promoter of tobacco mosaic virus. Chinese Journal of Virology 12(3): 243-253, 1996

Quantitation of HCV positive- and negative-strand RNA in liver tissue by strand-specific Taqman real-time PCR assays. Hepatology 38(4 Suppl 1): 435A, 2003

Alfalfa mosaic virus negative strand RNA synthesis by transiently expressed replicases. 2003

Template specificity of q beta phage and sp phage rna replicases as studied by replication of small variant rna. Molecular & General Genetics 169(2): 173-182, 1979

Replication of mitochondrial DNA occurs by strand displacement with alternative light-strand origins, not via a strand-coupled mechanism. Genes and Development 19(20): 2466-2476, 2005

Identification of negative strand and positive strand RNA of canine distemper virus in animal tissues using single stranded RNA probes. Journal of Virological Methods 18(2-3): 121-131, 1987