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Negative-strand RNA replication by Q beta and MS2 positive-strand RNA phage replicases

Negative-strand RNA replication by Q beta and MS2 positive-strand RNA phage replicases

Virology 178(1): 340-343

Plasmids were constructed which allowed preparation of positive- and negative-strand MS2 RNA by in vitro transcription and which expressed the MS2 phage replicase. Transcribed, positive-strand MS2 RNA was infectious to Escherichia coli spheroplasts. Transcribed, negative-strand MS2 RNA was infectious to spheroplasts containing either MS2 replicase or related bacteriophage Q beta replicase.

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Accession: 040779418

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PMID: 2202149

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