+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Neonatal W-mutant mice are favorable hosts for tracking development of marked hematopoietic stem cells



Neonatal W-mutant mice are favorable hosts for tracking development of marked hematopoietic stem cells



Experimental Hematology 17(8): 872-876



Neonatal unirradiated mice of W-mutant genotypes, with a hematopoietic stem cell defect and anemia, were injected i.v. with normal fetal liver hematopoietic cells. Efficient, long-term engraftment occurred as a result of the competitive advantage to the donor stem cells. The frequency of engraftment and rate of repopulation characteristically diminish in the series W/Wv, Wf/Wf, and Wv/+, in which the severity of the endogenous defect is progressively less. H-2 compatibility is required in the inbred strain combinations examined; other histocompatibility loci play a minor role in some strain combinations. Engraftment is due to self-renewing hematopoietic stem cells ancestral to myeloid and lymphoid lineages. The more mildly defective mutants display much greater variability in the kinetics of repopulation--a result consistent with seeding by single, or very few, stem cells that form developing clones. Engraftment efficiency is reduced by prolonged culture of fetal liver cells during experimental infection by recombinant retroviruses; nevertheless, after 24 h in vitro to achieve retroviral marking, stem cells retain their ability to repopulate and develop in W/Wv neonates.

Please choose payment method:






(PDF emailed within 1 workday: $29.90)

Accession: 040780299

Download citation: RISBibTeXText

PMID: 2569983


Related references

Clonal contributions of small numbers of retrovirally marked hematopoietic stem cells engrafted in unirradiated neonatal W/Wv mice. Proceedings of the National Academy of Sciences of the United States of America 86(12): 4564-4568, 1989

Hematopoietic cell clones in mice reconstituted with hematopoietic stem cells marked by lentiviral vector. Experimental Hematology (New York) 31(7 Suppl. 1): 191, 2003

In vivo clonal tracking of hematopoietic stem and progenitor cells marked by five fluorescent proteins using confocal and multiphoton microscopy. Journal of Visualized Experiments 2014(90): E51669, 2014

Transplantation of chromosomally marked syngeneic marrow cells into mice not subjected to hematopoietic stem cell depletion. Experimental Hematology 12(4): 277-283, 1984

Retrovirally marked human hematopoietic stem cells retained multilineage repopulating potentials in SCID-Hu mice. Blood 84(10 Suppl. 1): 344A, 1994

Tracking the origin, development, and differentiation of hematopoietic stem cells. Current Opinion in Cell Biology 49: 108-115, 2017

Tracking the origin, development, and differentiation of hematopoietic stem cells. Current Opinion in Cell Biology 49: 108-115, 2017

Engraftment efficacy of human hematopoietic stem cells transplanted into NOD/SCID mice using two methods: intra-bone marrow transplantation of hematopoietic stem cells and intravenous co-transplantation with mesenchymal stem cells. Acta Haematologica 131(3): 179-182, 2014

Hoxb4-deficient mice undergo normal hematopoietic development but exhibit a mild proliferation defect in hematopoietic stem cells. Blood 103(11): 4126-4133, 2004

Studies of W mutant mice provide evidence for alternate mechanisms capable of activating hematopoietic stem cells. Experimental Hematology 24(2): 185-194, 1996

In utero depletion of fetal hematopoietic stem cells improves engraftment after neonatal transplantation in mice. Blood 124(6): 973-980, 2014

The value of allogeneic and autologous hematopoietic stem cell transplantation in prognostically favorable acute myeloid leukemia with double mutant CEBPA. Blood 122(9): 1576-1582, 2013

In vivo transduction of hematopoietic stem cells after neonatal intravenous injection of an amphotropic retroviral vector in mice. Molecular Therapy 10(1): 37-44, 2004

Sequentially inducting murine embryonic stem cells into hematopoietic stem cells in vitro by hematopoietic development procedure for reconstitution of hematopoiesis in vivo. Zhongguo Shi Yan Xue Ye Xue Za Zhi 19(5): 1189-1194, 2011

Development of model for analysing respective collections of intended hematopoietic stem cells and harvests of unintended mature cells in apheresis for autologous hematopoietic stem cell collection. Transfusion and Apheresis Science 50(2): 294-302, 2014