+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Neonatal and adult 6-hydroxydopamine-induced lesions differentially alter tachykinin and enkephalin gene expression



Neonatal and adult 6-hydroxydopamine-induced lesions differentially alter tachykinin and enkephalin gene expression



Journal of Neurochemistry 49(5): 1623-1633



The present investigation examined the effects of neonatal and adult 6-hydroxydopamine (6-OHDA)-induced lesions of dopaminergic neurons on opioid and tachykinin peptides and their gene expression in the rat basal ganglia. This work was undertaken to determine if changes in these neuropeptide systems were contributing to the differing behavioral responses observed between neonatally and adult-lesioned rats after dopamine agonist administration. [Met5]Enkephalin (ME) content was increased in striatal tissue from both 6-OHDA-lesioned groups when compared with unlesioned controls. Dynorphin-A (1-8) content was not altered by the 6-OHDA lesions. The tachykinin peptides substance P and neurokinin A were significantly decreased in level in the striatum and substantia nigra of neonatally lesioned rats, but not in the adult-lesioned rats, when compared with unlesioned controls. Proenkephalin mRNA abundance (quantified by an RNA-cDNA hybridization technique) and precursor level (as reflected by cryptic ME content) were increased in the striatum of both neonatally and adult-lesioned rats. The abundance of preprotachykinin mRNA coding for the tachykinin peptides was markedly decreased in the neonatally lesioned rats, whereas only a small reduction was observed in the adult-lesioned rats. These results suggest that destruction of dopamine-containing terminals with 6-OHDA elevates the level of ME by accelerating transcriptional and/or translational processes; conversely, the reduced content of tachykinins in neonatally lesioned rats may be due to a reduction in such processes. Thus, preproenkephalin-A and preprotachykinin gene expression are differentially regulated after lesioning of catecholamine-containing neurons, an observation suggesting a close functional relationship among these neurotransmitter systems. Furthermore, of the peptides studied, only levels of the tachykinin peptides were differentially altered in the striatum and substantia nigra of the neonatally lesioned rats compared with adult-lesioned rats; therefore, these peptides may be associated with the distinctive behavioral differences between neonatally and adult 6-OHDA-lesioned rats given dopamine agonists.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 040780365

Download citation: RISBibTeXText

PMID: 2889804

DOI: 10.1111/j.1471-4159.1987.tb01036.x


Related references

Lesions of hypothalamic paraventricular nuclei do not prevent the effect of estradiol on energy and fat balance. American Journal Of Physiology. 267(1 Part 1): E32-E38, 1994

Neonatal cortical and thalamic lesions disrupt the normal pattern of projection neurons but not primary afferent axons in rat trigeminal nucleus principalis. Society for Neuroscience Abstracts 16(2): 1152, 1990

Stimulated secretion of pituitary hormones in normal humans: A novel direct assessment from blood concentrations. Annals of Biomedical Engineering 28(9): 1136-1145, 2000

Motor functions of the bat frontal auditory field. European Journal of Neuroscience 12(Suppl. 11): 131, 2000

Motor activity (exploration) and formation of home bases in mice (C57BL/6) influenced by visual and tactile cues: modification of movement distribution, distance, location, and speed. Physiology and Behavior 87(4): 805-816, 2006

Nigral gamma vinyl gaba infusions and flurothyl induced seizures developmental effects in rats. Society for Neuroscience Abstracts 17(1-2): 1607, 1991

Left Ventricular Thrombus in Cardiomyopathy: Echocardiographic Assessment. Acta Medica Nagasakiensia 39(4): 168-171, 1994

Effects of neomycin on the development of tolerance to morphine antinociception. Life Sciences 69(18): 2081-2090, 2001

Neonatal 6-OHDA lesions alter the developmental expression of striatal D1 D1 receptors and D1 mRNA. Society for Neuroscience Abstracts 20(1-2): 1299, 1994

Selective D-1 dopamine receptor increase following chronic treatment with SCH 23390. European Journal of Pharmacology 109(1): 127-128, 1985

Ultrastructure of Merkel-like cells labeled with carbocyanine dye in the non-taste lingual epithelium of the axolotl. Neuroscience Letters 178(1): 1-4, 1994

Neonatal lesion of forebrain cholinergic neurons: further characterization of behavioral effects and permanency. Neuroscience 133(2): 485-492, 2005

Cholinergic regulation of rat preproenkephalin RNA in the adrenal medulla. Molecular Brain Research. 18(1-2): 133-140, 1993