+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Normal chemotactic migration of polymorphonuclear leukocytes stimulated with mononuclear-derived chemotactic factor in ulcerative colitis



Normal chemotactic migration of polymorphonuclear leukocytes stimulated with mononuclear-derived chemotactic factor in ulcerative colitis



International Archives of Allergy and Applied Immunology 81(1): 63-68



The migration of polymorphonuclear leukocytes preincubated with autologous or heterologous serum was examined in 100 patients with untreated ulcerative colitis (UC) and in 100 age- and sex-matched healthy controls. The activity of complement-derived chemotactic factors and mononuclear-derived chemotactic factor (MDCF) was also investigated in the same group of patients. No significant difference was found in random and chemotactic migration of patients or control leukocytes preincubated in different concentrations (10, 50 and 100%) of autologous or heterologous serum. Defective chemotaxis of leukocytes stimulated with complement-derived chemotactic factors was found in UC and was more marked in patients in remission than with active UC independently of whether complement was activated by the alternative or the classical pathway. However, the random migration of neutrophils was enhanced in both groups of UC patients. The leukocytes of patients stimulated with MDCF (mononuclear cells were activated with lipopolysaccharide from Escherichia coli O55:B5) show normal chemotaxis. Our data suggest an impairment of neutrophil receptors for complement-derived chemotactic factor in UC. The decreased response of neutrophils to these factors and normal response to MDCF suggest that the main way in which cells are attracted to the site of inflammation in UC may be a factor produced by stimulated mononuclear cells.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 040820524

Download citation: RISBibTeXText

PMID: 3744579

DOI: 10.1159/000234109


Related references

The relationship of the chemotactic behavior of the complement-derived factors, C3a, C5a, and C567, and a bacterial chemotactic factor to their ability to activate the proesterase 1 of rabbit polymorphonuclear leukocytes. Journal of Experimental Medicine 135(2): 376-387, 1972

Chemotactic factor to polymorphonuclear leukocytes in the crystallin lens 1. age related chemotactic activity by chemotaxis assay. Nippon Ganka Gakkai Zasshi 89(2): 317-322, 1985

Chemotactic factor to polymorphonuclear leukocytes in the human lens 6. chemotactic factor in low molecular weight fractions. Nippon Ganka Gakkai Zasshi 90(8): 1110-1114, 1986

Novel neutrophil chemotactic factor derived from human peripheral blood mononuclear leukocytes. Clinical & Experimental Immunology 64(1): 214-222, 1986

The influence of mononuclear leukocytes on the chemotactic responsiveness of polymorphonuclear leukocytes of bovines. Journal of Veterinary Medicine Series B 37(5): 332-336, 1990

Chemotactic factor to polymorphonuclear leukocytes in the human lens 7. chemotactic factor leaked through lens capsule. Nippon Ganka Gakkai Zasshi 90(12): 1640-1645, 1986

Chemotactic factor to polymorphonuclear leukocytes in the human crystallin lens. 5. Chemotactic factor in gamma-crystallin. Nippon Ganka Gakkai Zasshi 90(1): 167-172, 1986

The relative responsiveness of mononuclear leukocytes and polymorphonuclear leukocytes to bacterial and serum chemotactic factors. Federation Proceedings 30(2): 355, 1971

Chemotactic factor for polymorphonuclear leukocytes derived from human lung giant cell carcinoma lu 65 cells. Journal of Clinical Biochemistry & Nutrition 6(2): 147-154, 1989

Polymorphonuclear granulocyte chemotaxis and chemotactic factor generation by concanavalin A-stimulated peripheral blood mononuclear cells in patients with psoriasis. Archives of Dermatological Research 271(3): 259-264, 1981

Neutrophil chemotactic factor produced by lipopolysaccharide lps stimulated human mononuclear leukocytes partial characterization and separation from interleukin 1 il 1. Federation Proceedings 46(3): 735, 1987

Chemotactic responses of normal human basophils to C5a and to lymphocyte-derived chemotactic factor. Journal of Immunology 117(1): 246-252, 1976

Neutrophil chemotactic factor produced by lipopolysaccharide (LPS)-stimulated human blood mononuclear leukocytes: partial characterization and separation from interleukin 1 (IL 1). Journal of Immunology 139(3): 788-793, 1987

Regulation of Receptors for Formyl-Chemotactic Peptide in Human Polymorphonuclear Leukocytes: Analysis of Chemotactic Dysfunction of Cord Blood Granulocytes. Pediatrics International 27(4): 552-556, 1985

Neutrophil chemotactic factor produced by lipopolysaccharide (LPS)-stimulated human blood mononuclear leukocytes: partial characterization and separation from interleukin 1 (IL 1). 1987. Journal of Immunology 175(9): 5569-5574, 2005