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On-line high-performance liquid chromatography for the determination of cephalosporin C and by-products in complex fermentation broths


On-line high-performance liquid chromatography for the determination of cephalosporin C and by-products in complex fermentation broths



Journal of Chromatography 499: 609-615



ISSN/ISBN: 0021-9673

PMID: 2324218

DOI: 10.1016/s0021-9673(00)97005-5

A fully automated high-performance liquid chromatographic system was developed for optimization of cephalosporin C production. Using a simple, isocratic, reversed-phase method, cephalosporin C, deacetylcephalosporin C, deacetoxycephalosporin C, penicillin N, methionine and its decomposition product 2-hydroxy-4-methylmercaptobutyric acid were determined. The method worked reliably for 250 h and supplied information on the influence of many parameters of the process.

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Accession: 040863649

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Related references

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