+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Oxaloacetate permeation in rat kidney mitochondria: pyruvate/oxaloacetate and malate/oxaloacetate translocators



Oxaloacetate permeation in rat kidney mitochondria: pyruvate/oxaloacetate and malate/oxaloacetate translocators



Biochemical and Biophysical Research Communications 129(1): 1-10



The mechanism of oxaloacetate efflux from rat kidney mitochondria has been investigated in view of its possible role both in gluconeogenesis and in transferring cytosolic reducing equivalents into mitochondria. Thus reconstruction of the malate/oxaloacetate shuttle made possible by the oxaloacetate carrier has been made. Moreover the existence of a separate translocator able to allow a bidirectional alpha-cyanocinnamate-insensitive pyruvate/oxaloacetate exchange has been ascertained. This carrier is specific of gluconeogenetic organs in particularly of kidney, where it shows a marked affinity for pyruvate (Km = 0.45 mM and Vmax = 38 nmoles oxaloacetate effluxed/min X mg mitochondrial protein at 20 degrees C). Some features of both pyruvate/oxaloacetate and malate/oxaloacetate exchanges are also described.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 040895764

Download citation: RISBibTeXText

PMID: 4004869

DOI: 10.1016/0006-291x(85)91394-4


Related references

Oxaloacetate uptake into rat brain mitochondria and reconstruction of the malate/oxaloacetate shuttle. Biochemical and Biophysical Research Communications 119(3): 1039-1046, 1984

Regulation of malate oxidation in isolated mung bean mitochondria: I. Effects of oxaloacetate, pyruvate, and thiamine pyrophosphate. Plant Physiology 58(3): 433-437, 1976

Oxaloacetate Decarboxylation and Oxaloacetate-Carbon Dioxide Exchange in Acetobacter Xylinum. Journal of Bacteriology 88: 1678-1687, 1964

The role of biotin and sodium in the decarboxylation of oxaloacetate by the membrane-bound oxaloacetate decarboxylase from Klebsiella aerogenes. European Journal of Biochemistry 121(2): 435-441, 1982

L-Lactate metabolism in HEP G2 cell mitochondria due to the l-lactate dehydrogenase determines the occurrence of the lactate/pyruvate shuttle and the appearance of oxaloacetate, malate and citrate outside mitochondria. Biochimica et Biophysica Acta 1817(9): 1679-1690, 2012

The permeability of mitochondria to oxaloacetate and malate. Biochemical Journal 107(5): 659-667, 1968

The generation of an electrochemical gradient of sodium ions upon decarboxylation of oxaloacetate by the membrane-bound and Na+-activated oxaloacetate decarboxylase from Klebsiella aerogenes. European Journal of Biochemistry 121(2): 443-449, 1982

The rates of penetration of oxaloacetate and L-malate into mitochondria. Biochemical Journal 104(3): 51p-52p, 1967

Oxaloacetate and malate transport by plant mitochondria. Plant Physiology 87(1): 109-115, 1988

Studies on malate or oxaloacetate formation from bicarbonate and pyruvate or phosphoenol pyruvate in cell-free extracts of Leishmania donovani. Comparative Biochemistry and Physiology. B, Comparative Biochemistry 47(3): 725-738, 1974

Factors affecting the penetration of oxaloacetate and L-malate into rat-liver mitochondria. Biochemical Journal 104(3): 52p, 1967

Nucleotide effects on the oxidation of malate and oxaloacetate by Arum mitochondria. New phytologist: 76 (2) 213-218, 1976

The inhibition of malate oxidation by oxaloacetate in Jerusalem artichoke mitochondria. Developments in plant biology(1): 117-124, 1978

Factors affecting the translocation of oxaloacetate and L-malate into rat liver mitochondria. Biochemical Journal 109(5): 921-928, 1968

Effect of bicarbonate and oxaloacetate on malate oxidation by spinach leaf mitochondria. Biochimica et Biophysica Acta 589(2): 176-189, 1980