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Partial purification and characterization of B cell growth factor constitutively secreted by human T-cell hybridoma



Partial purification and characterization of B cell growth factor constitutively secreted by human T-cell hybridoma



Immunology Letters 10(6): 339-346



Human T cell hybridomas were established by fusion of PHA-activated PBL with the 8-azaguanine resistant human T-leukemic cell line CEM-CM3. High levels of B cell growth factor (BCGF) activity were detected in the supernatants of hybridoma C8-2B2 and its subclones. Hybridoma C8-2B2, in addition to the Leu 3a, also expressed the OKT11 surface marker which was not detectable on the parent CEM-CM3 cells. BCGF from the culture supernatant was purified by combined use of salt fractionation and gel filtration to 36.6 fold with 23.9% recovery of activity. The BCGF produced by hybridoma C8-2B2 has a molecular weight range of 16,000-20,000 in two major electrophoretically different forms with pI values of 6.4 and 7.4.

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Accession: 040914296

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PMID: 3930398

DOI: 10.1016/0165-2478(85)90129-4


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