Section 41
Chapter 40,955

Phage lambda receptor chromosomes for DNA fragments made with restriction endonuclease i of Bacillus amyloliquefaciens H

Klein, B.; Murray, K.

Journal of Molecular Biology 133(2): 289-294


ISSN/ISBN: 0022-2836
PMID: 231662
DOI: 10.1016/0022-2836(79)90537-0
Accession: 040954420

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A lambda derivative with Dna resistant to attack by R. Bam Hi has been obtained following mutagenesis to remove a single target for this enzyme. The two central targets for R. Bam Hi were then introduced into the genome of this phage and the Dna between them removed in vitro to give a lambda insertion vector for fragments of Dna produced by digestion with R. Bam HI.

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