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Platelet plasminogen activator inhibitor: purification and characterization of interaction with plasminogen activators and activated protein C


, : Platelet plasminogen activator inhibitor: purification and characterization of interaction with plasminogen activators and activated protein C. Biochemistry 28(14): 5773-5778

Plasminogen activator inhibitor (PAI) was purified in active form from porcine platelets under nondenaturing conditions. The purified inhibitor (Mr 47,000) reacts with tissue-type plasminogen activator (t-PA), urokinase (UK), and activated protein C (APC) to yield both SDS-stable complexes and a modified PAI of slightly reduced molecular weight. The second-order rate constants for the inhibition of t-PA and UK by PAI are 3.5 X 10(7) and 3.4 X 10(7) M-1 s-1, respectively. Activated protein C reacts with PAI with a second-order rate constant of 1.1 X 10(4) M-1 s-1. This rate is not accelerated by protein S, phospholipid, and calcium, or heparin. It is concluded that (1) PAI can function as both inhibitor and substrate of its target proteases, (2) if APC promotes fibrinolysis via inactivation of PAI, then APC must be present in concentrations several orders of magnitude greater than t-PA, or the interaction of APC and PAI must be accelerated by presently unknown mechanisms, and (3) in the absence of heparin, platelet PAI is the most rapid inhibitor of APC yet described.

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Accession: 040996116

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Fisch M.M.; Riedmiller H.N.; Hohenfellner R., 1989: Boari hitch technique and ileoileal bladder augmentation in replacement of long distal ureteral lesions. Journal of Urology 141(4 PART 2): 377A

Mase, Y., 1959: Biosynthesis of carotenoids by fungi. Penicillium sclerotiorum, P. multicolor, P. lapidosum and Rhizoctonia zeae were found to produce carotenoids when grown on Difco wort agar media. The carotenoids were extracted, separated, identified and estimated. P. sclerotiorum NRRL 2074 gave m...

Rubio Paredes, J., 1954: Use of penicillin mycelium in the feeding of birds. From analysis of 50 samples of dried penicillin mycelium, the mean composition, per cent., was moisture 3.4, crude protein 31.3, crude fat 4.6, N-free extract 22.2, crude fibre 9.7 and ash 28.8. The mean riboflavin content was 40.25 micro g. per g...

Hamel, PB., 1977: The wood stork in South Carolina, a review. Chat, 412: 24-27

Roos, M. T. L.; Koot, M.; Tersmette, M.; Keet, I.; Coutinho, R. A.; Miedema, F.; Schellekens, P. T. A., 1993: T cell function and HIV SI phenotype are prognostic markers for development of AIDS in HIV-1-infected homosexual men. IXTH INTERNATIONAL CONFERENCE ON AIDS AND THE IVTH STD WORLD CONGRESS [Author] IXth International Conference on AIDS in affiliation with the IVth STD World Congress : 44

von Frese Ralph R. B.; Roman Daniel R.; Kim Jeong Hee; Kim Jeong Woo; Anderson Allen J., 1999: Satellite mapping of the Antarctic gravity field. The production and analysis of the Antarctic digital magnetic anomaly map will be greatly aided by complementary gravity data. They help to constrain thickness variations of the crust and related magnetic effects that may be used for correcting lo...

Hillidge, Cj; Lees, P; Serrano, L., 1973: Investigations of azaperone. Veterinary record: 5, 93 (11) 307-311

Witucki, L., 1988: U.S. broiler exports set record in 1987. Agricultural outlook AO United States Deptartment of Agriculture Economic Research Service: (141) 15-16

Marschall H U.; Wietholtz H.; Dressen M.; Reuschenbach R.; Matern H.; Matern S.; Sjovall J., 1989: Glycosylation of chenodeoxycholic hyodeoxycholic and ursodeoxycholic acids in cholestatic patients. Journal of Hepatology 9(SUPPL 1): S191

Richter, G. A., 1941: Cellulose from hardwoods: wood pulp purification. Unbleached hardwood pulps can be processed to yield substantially white products that possess properties and chemical composition suitable for esterification and for conversion into stable papers. Means are given for the elimination of resinous bo...