Pregnancies after co-culture of cumulus cells with bovine embryos derived from in-vitro fertilization of in-vitro matured follicular oocytes
Goto, K.; Kajihara, Y.; Kosaka, S.; Koba, M.; Nakanishi, Y.; Ogawa, K.
Journal of Reproduction and Fertility 83(2): 753-758
ISSN/ISBN: 0022-4251 PMID: 3411565 DOI: 10.1530/jrf.0.0830753
Bovine follicular oocytes surrounded by cumulus cells for more than one-third of their surface were matured, fertilized and developed in vitro utilizing a co-culture system with bovine cumulus cells. Embryos developed into blastocysts were non-surgically transferred to the uteri of cows at Day 6, 7 or 8 (Day 0 = oestrus). Out of 6 recipient cows (19 blastocysts transferred), 3 became pregnant. One of the 3 pregnant cows carried twins. The results of this study demonstrated the viability of embryos obtained from in-vitro maturation of bovine oocytes followed by in-vitro fertilization and culture to the blastocyst stage in vitro.