Section 42
Chapter 41,036

Pregnancies after co-culture of cumulus cells with bovine embryos derived from in-vitro fertilization of in-vitro matured follicular oocytes

Goto, K.; Kajihara, Y.; Kosaka, S.; Koba, M.; Nakanishi, Y.; Ogawa, K.

Journal of Reproduction and Fertility 83(2): 753-758


ISSN/ISBN: 0022-4251
PMID: 3411565
DOI: 10.1530/jrf.0.0830753
Accession: 041035844

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Bovine follicular oocytes surrounded by cumulus cells for more than one-third of their surface were matured, fertilized and developed in vitro utilizing a co-culture system with bovine cumulus cells. Embryos developed into blastocysts were non-surgically transferred to the uteri of cows at Day 6, 7 or 8 (Day 0 = oestrus). Out of 6 recipient cows (19 blastocysts transferred), 3 became pregnant. One of the 3 pregnant cows carried twins. The results of this study demonstrated the viability of embryos obtained from in-vitro maturation of bovine oocytes followed by in-vitro fertilization and culture to the blastocyst stage in vitro.

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