+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Presence of genomic polyadenylate and absence of detectable virion transcriptase in human coronavirus OC-43

Presence of genomic polyadenylate and absence of detectable virion transcriptase in human coronavirus OC-43

Journal of General Virology 39(1): 29-39

Human coronavirus RNA, prepared by extraction of purified virions with phenol-chloroform, consists of a major 15 to 55S class and a minor 4S class of RNA fragments. Polyadenylic acid [poly (A)] sequences are present in 15 to 55S but not in 4S RNA, suggesting different functions for each class. A stretch of poly (A) of approximately 19 adenosine monophosphate residues was obtained in sizing experiments after digesting OC-43 RNA with pancreatic and T1 ribonucleases. An OC-43 virion RNA transcriptase could not be detected with systems optimal for detecting the transcriptases of influenza and Newcastle disease virus.

Please choose payment method:

(PDF emailed within 1 workday: $29.90)

Accession: 041048686

Download citation: RISBibTeXText

PMID: 641531

Related references

Characterization of an HIV-1 isolate displaying an apparent absence of virion-associated reverse transcriptase activity. Aids Research and Human Retroviruses 7(3): 295-302, 1991

Genomic relationship of porcine hemagglutinating encephalomyelitis virus to bovine coronavirus and human coronavirus OC43 as studied by the use of bovine coronavirus S gene-specific probes. Archives of virology40(7): 1215-1223, 1995

Equilibrium constant and dissociation rate of human immunodeficiency virus reverse transcriptase in the presence and absence of Mg+2. Abstracts of the General Meeting of the American Society for Microbiology 99: 629-630, 1999

An interaction between the nucleocapsid protein and a component of the replicase-transcriptase complex is crucial for the infectivity of coronavirus genomic RNA. Journal of Virology 84(19): 10276-10288, 2010

The raft-promoting property of virion-associated cholesterol, but not the presence of virion-associated Brij 98 rafts, is a determinant of human immunodeficiency virus type 1 infectivity. Journal of Virology 78(19): 10556-10565, 2004

Complete genomic sequence of human coronavirus OC43: molecular clock analysis suggests a relatively recent zoonotic coronavirus transmission event. Journal of Virology 79(3): 1595-1604, 2005

Genotypic and phenotypic changes during culture of a multinucleoside-resistant human immunodeficiency virus type 1 strain in the presence and absence of additional reverse transcriptase inhibitors. Antimicrobial Agents and ChemoTherapy 40(12): 2887-2890, 1996

Sendai virion transcriptase complex: polyeptide composition and inhibition by virion envelope proteins. Journal of Virology 13(1): 107-112, 1974

Absence of detectable anti hcv c 100 in the presence of other hcv specific antibodies. Transfusion 31(8 Suppl.): 12S, 1991

Polyadenylate in the virion RNA of mouse hepatitis virus. Journal of Biochemistry 82(4): 1103-1108, 1977

Genomic sequencing reveals absence of DNA methylation in the major late promoter of adenovirus type 2 DNA in the virion and in productively infected cells. Febs Letters. 362(3): 301-305, 1995