+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Preservation of Xenopus laevis rDNA-containing plasmid, pXlr101A, injected into the fertilized egg of Xenopus laevis

Preservation of Xenopus laevis rDNA-containing plasmid, pXlr101A, injected into the fertilized egg of Xenopus laevis

Cell Structure and Function 11(2): 109-114

A circular rDNA-containing recombinant plasmid, pXlr101A, and its vector pBR322 were microinjected into the cytoplasm of fertilized Xenopus laevis eggs. The DNAs extracted from injected embryos at various stages of development were analyzed by hybridization with 32P-labeled pBR322 as the probe. Neither pXlr101A nor pBR322 were replicated, but they were maintained until the tailbud stage, disappearing during the muscular response stage. When pXlr101A-injected embryos were raised until the 2-week-old tadpole stage, sequences homologous to pBR322 were detectable in two Eco RI fragments of the pXlr101A-injected tadpole DNA. The sizes of the Eco RI fragments suggested that the plasmid sequences were preserved most probably in the chromosome-integrated form.

Please choose payment method:

(PDF emailed within 1 workday: $29.90)

Accession: 041050640

Download citation: RISBibTeXText

PMID: 3731283

Related references

Preservation of xenopus laevis ribosomal dna containing plasmid pxlr 101a injected into the fertilized egg of xenopus laevis. Cell Structure & Function 11(2): 109-114, 1986

Differential expression of the Xenopus laevis tadpole and adult beta-globin genes when injected into fertilized Xenopus laevis eggs. Molecular and Cellular Biology 4(3): 567-570, 1984

Replication and expression of Xenopus laevis globin genes injected into fertilized Xenopus eggs. Proceedings of the National Academy of Sciences of the United States of America 80(20): 6197-6201, 1983

Chromatin specific hypersensitive sites are assembled on a xenopus laevis histone gene injected into xenopus laevis oocytes. Journal of Molecular Biology 181(3): 333-350, 1985

Fidelity of transcription of Xenopus laevis globin genes injected into Xenopus laevis oocytes and unfertilized eggs. Molecular and Cellular Biology 4(10): 2109-2119, 1984

Transcription of xenopus laevis methionyl transfer dna 1 and sea urchin histone dna injected into the xenopus laevis oocyte nucleus. Cold Spring Harbor Lab Cold Spring Harbor Symposia on Quantitative Biology, Vol 42 Chromatin Part 1 And 2 Cold Spring Harbor, N Y , Usa, June 1-8, 1977 Xxii+603p (Part 1); Xxii+657p (Part 2) Cold Spring Harbor Laboratory: Cold Spring Harbor, N Y , Usa Isbn 0-87969-041-0 1077-1082, 1978

Nuclear transplantations between two subspecies of Xenopus laevis, (Xenopus laevis laevis and Xenopus laevis petersi). Acta Embryologiae et Morphologiae Experimentalis 9(2): 187-202, 1966

Development of the thyroid metabolism of radio iodide during metamorphosis in 2 species of xenopus xenopus laevis laevis and xenopus clivii. Compte Rendu des Seances de la Societe de Physique et d'Histoire Naturelle de Geneve 11(1-3): 45-52, 1976

Preferential transcription of Xenopus laevis ribosomal RNA in interspecies hybrids between Xenopus laevis and Xenopus mulleri. Journal of Molecular Biology 80(2): 217-228, 1973

Formation of nucleus like structure by exogenous dna injected into xenopus laevis fertilized eggs. Cell Structure & Function 11(4): 467, 1986

Replication, integration and expression of exogenous DNA injected into fertilized eggs of Xenopus laevis. Differentiation; Research in Biological Diversity 26(3): 194-202, 1984

Replication, methylation, and expression of X laevis globin genes injected into fertilized Xenopus eggs. Progress in Clinical and Biological Research 134: 27-38, 1983

Regional accumulation of vegetal pole poly adenylated rna injected into fertilized xenopus laevis eggs. Nature (London) 294(5838): 255-257, 1981

The translation of mammalian globin mRNA injected into fertilized eggs of Xenopus laevis I. Message stability in development. Developmental Biology 39(1): 125-133, 1974

The external transcribed spacer and preceding region of Xenopus borealis rDNA: comparison with the corresponding region of Xenopus laevis rDNA. Nucleic Acids Research 11(23): 8183-8196, 1983