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Primary structure and function of novel O-glycosylated hirudins from the leech Hirudinaria manillensis



Primary structure and function of novel O-glycosylated hirudins from the leech Hirudinaria manillensis



Biochemistry 31(8): 2294-2298



Hirudin from the leech Hirudo medicinalis is a most powerful anticoagulant, and many isoforms have been described. In the present work, the primary structure of two hirudins from the leech Hirudinaria manillensis has been elucidated. The antithrombotic activity is similar to that of H. medicinalis hirudins although the sequence identity is below 60%. Surprisingly, the hirudins were found to be glycosylated at one site. Sugar analysis after methanolysis yielded fucose, galactose, and N-acetylgalactosamine. These results combined with data from matrix-assisted laser desorption ionization mass spectrometry, plasma desorption mass spectrometry, capillary zone electrophoresis, and lectin-binding tests indicate that the sequence is Fuc-Gal beta 1-3GalNAc-(O-threonine). This structure shows an interesting similarity to human blood group H determinants.

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Accession: 041068817

Download citation: RISBibTeXText

PMID: 1540584

DOI: 10.1021/bi00123a012


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